Hammami Inés, Smaoui Slim, Hsouna Anis Ben, Hamdi Naceur, Triki Mohamed Ali
Unité de Recherche Protection des Plantes Cultivées et Environnement, Institut de l'Olivier, Sfax BP1087, Tunisia.
Laboratoire de Microorganismes et de Biomolécules du Centre de Biotechnology de Sfax. Route Sidi Mansour Km 6 B.P. 117, 3018 Sfax- Tunisia.
EXCLI J. 2015 Jan 13;14:83-94. doi: 10.17179/excli2014-655. eCollection 2015.
The aims of this study were to assess the antimicrobial efficacy of the leaf essential oil and the leaf extracts of R. montana against Botrytis cinerea, Fusarium oxysporum, Verticillium dahliae, Aspergillus oryzae and Fusarium solani. The oil (1.000 µg/disk) and the extracts (1.500 µg/disk) revealed a remarkable antifungal effect against the tested plant pathogenic fungi with a radial growth inhibition percentage of 40.0-80.0 % and 5.0-58.0 %, respectively along with their respective MIC values ranging from 100 to 1100 µg/mL and 250 to 3000 µg/mL. The oil had a strong detrimental effect on spore germination of all the tested plant pathogens along with the concentration as well as time-dependent kinetic inhibition of Fusarium oxysporum. Also, the oil exhibited a potent in vivo antifungal effect against Botrytis cinerea on tomato plants. Experiments carried out in plant revealed that the essential oil was slightly effective in suppression of gall formation induced by Agrobacterium tumefaciens on bitter almond. The results of this study indicate that the oil and extracts of R. montana leaves could become natural alternatives to synthetic fungicides to control certain important plant microbial diseases. The GC-MS analysis determined that 28 compounds, which represented 89.03 % of total oil, were present in the oil containing mainly 1-butene, methylcyclopropane, 2-butene and caryophyllene oxide.
本研究的目的是评估山地玫瑰叶精油和叶提取物对灰葡萄孢菌、尖孢镰刀菌、大丽轮枝菌、米曲霉和茄类镰刀菌的抗菌效果。该精油（1.000微克/圆盘）和提取物（1.500微克/圆盘）对受试植物病原真菌显示出显著的抗真菌作用，径向生长抑制率分别为40.0 - 80.0%和5.0 - 58.0%，其各自的最低抑菌浓度值范围为100至1100微克/毫升和250至3000微克/毫升。该精油对所有受试植物病原体的孢子萌发有强烈的有害作用，同时对尖孢镰刀菌有浓度和时间依赖性的动力学抑制作用。此外，该精油对番茄植株上的灰葡萄孢菌表现出有效的体内抗真菌作用。在植物上进行的实验表明，该精油在抑制根癌土壤杆菌诱导的苦杏仁上的瘤形成方面效果稍差。本研究结果表明，山地玫瑰叶精油和提取物可成为控制某些重要植物微生物病害的合成杀菌剂的天然替代品。气相色谱 - 质谱分析确定该精油中存在28种化合物，占总油含量的89.03%，主要包含1 - 丁烯、甲基环丙烷、2 - 丁烯和氧化石竹烯。
