通过偏振二次谐波产生显微镜观察甲状腺癌组织中胶原蛋白的超微结构特征。
Ultrastructural features of collagen in thyroid carcinoma tissue observed by polarization second harmonic generation microscopy.
作者信息
Tokarz Danielle, Cisek Richard, Golaraei Ahmad, Asa Sylvia L, Barzda Virginijus, Wilson Brian C
机构信息
Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto, ON M5G 1L7, Canada.
Department of Physics, Institute for Optical Sciences and Department of Physical and Chemical Sciences, University of Toronto, 3359 Mississauga Road North, Mississauga, ON L5L 1C6, Canada.
出版信息
Biomed Opt Express. 2015 Aug 21;6(9):3475-81. doi: 10.1364/BOE.6.003475. eCollection 2015 Sep 1.
Abstract
Changes in collagen ultrastructure between malignant and normal human thyroid tissue were investigated ex vivo using polarization second harmonic generation (SHG) microscopy. The second-order nonlinear optical susceptibility tensor component ratio and the degree of linear polarization (DOLP) of the SHG signal were measured. The ratio values are related to the collagen ultrastructure, while DOLP indicates the relative amount of coherent signal and incoherent scattering of SHG. Increase in ratio values and decrease in DOLP were observed for tumor tissue compared to normal thyroid, indicating higher ultrastructural disorder in tumor collagen.
摘要
利用偏振二次谐波产生（SHG）显微镜在体外研究了人甲状腺恶性组织与正常组织之间胶原蛋白超微结构的变化。测量了SHG信号的二阶非线性光学极化率张量分量比和线性偏振度（DOLP）。比值与胶原蛋白超微结构有关，而DOLP表示SHG的相干信号和非相干散射的相对量。与正常甲状腺相比，肿瘤组织的比值增加而DOLP降低，表明肿瘤胶原蛋白的超微结构紊乱程度更高。
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