Taktaz Hafshejani Taghi, Nekoei Shahin, Vazirian Behnam, Doosti Abbas, Khamesipour Faham, Anyanwu Madubuike Umunna
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
Biomed Res Int. 2015;2015:917854. doi: 10.1155/2015/917854. Epub 2015 Sep 1.
This study was undertaken to investigate molecularly the occurrence of EHV-1 and EHV-4 infection among equine population in regions, Iran. Blood samples from 53 and 37 randomly selected horses settled in Isfahan and Shahrekord, Iran, respectively, were collected. Detection of EHV-1 and EHV-4 genes in the blood samples was done using polymerase chain reaction (PCR). Out of 53 and 37 samples from Isfahan and Shahrekord, 4 (18.18%) and 3 (8.10%) were positive for PCR of EHV-1, respectively. Nine (16.98%) and 6 (16.21%) were positive for PCR of EHV-4, while 6 (11.32%) and 3 (8.10%) were positive for PCR of both EHV-1 and EHV-4, in Isfahan and Shahrekord, respectively. Of the 7 blood samples positive for EHV-1, 4 (16.66%) and 3 (8.10%) were from horses >3 years old while 2 (18.18%) and 1 (16.66%) were from 2-3 years old horses, in Isfahan and Shahrekord, respectively. Out of the 7 and 3 samples positive for PCR of EHV-1 in Isfahan and Shahrekord, 4 (22.2%) and 1 (7.69%) were Standardbred, while 3 (14.28%) and 2 (13.33%) were Thoroughbreds, respectively. EHV-4 was detected in blood of 4 (22.22%) and 2 (15.83%) Standardbreds and from 4 (19.04%) and 4 (26.66%) Thoroughbred horses in Isfahan and Shahrekord, respectively. This study has shown that horses settled in Isfahan central and Shahrekord southwest regions, Iran, are infected by EHV-1 and EHV-4 and thus serve as potential reservoirs and disseminators of the viruses.
本研究旨在从分子层面调查伊朗各地区马群中EHV - 1和EHV - 4感染的发生情况。分别从伊朗伊斯法罕和沙赫鲁德随机选取53匹和37匹马采集血样。使用聚合酶链反应（PCR）检测血样中的EHV - 1和EHV - 4基因。在来自伊斯法罕的53份样本和沙赫鲁德的37份样本中，EHV - 1的PCR检测分别有4份（18.18%）和3份（8.10%）呈阳性。EHV - 4的PCR检测分别有9份（16.98%）和6份（16.21%）呈阳性，而在伊斯法罕和沙赫鲁德，同时感染EHV - 1和EHV - 4的PCR检测分别有6份（11.32%）和3份（8.10%）呈阳性。在7份EHV - 1呈阳性的血样中，来自伊斯法罕和沙赫鲁德年龄大于3岁的马分别有4份（16.66%）和3份（8.10%），年龄在2 - 3岁的马分别有2份（18.18%）和1份（16.66%）。在伊斯法罕和沙赫鲁德EHV - 1的PCR检测呈阳性的7份和3份样本中，标准赛马分别有4份（22.2%）和1份（7.69%），纯种马分别有3份（14.28%）和2份（13.33%）。在伊斯法罕和沙赫鲁德，分别在4份（22.22%）和2份（15.83%）标准赛马以及4份（19.04%）和4份（26.66%）纯种马的血液中检测到EHV - 4。本研究表明，定居在伊朗伊斯法罕中部和沙赫鲁德西南部地区的马感染了EHV - 1和EHV - 4，因此是这些病毒的潜在宿主和传播者。
