Neumann A P, Suen G
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.
J Appl Microbiol. 2015 Dec;119(6):1515-26. doi: 10.1111/jam.12960. Epub 2015 Nov 9.
The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota.
454 pyrosequencing of the V6-V8 region of the 16S rRNA gene and quantitative PCR were employed to examine the bacterial microbiota and Clostridium perfringens, respectively, in the jejunum and caecum of market-age broiler chickens over four replicate grow-outs.
Our results suggest that virginiamycin has a more pronounced impact on broiler gastrointestinal tract bacterial communities, relative to BMD, manifested primarily through significant enrichments in the genus Faecalibacterium in the caecum and a distinct population of Lactobacillus, OTU_02, in both the jejunum and caecum. No evidence for a difference among the diets in Cl. perfringens levels in the jejunum or caecum was observed.
This work represents the highest resolution comparison to date of the jejunum and caecum microbiota in broilers fed either virginiamycin or BMD, and provides evidence for specific bacterial OTUs potentially involved in the health and performance benefits typically attributed to these in-feed antibiotics.
本研究旨在比较在美国商业家禽生产者中常用的两种饲料抗生素——维吉尼亚霉素和杆菌肽亚甲基二水杨酸酯（BMD）对鸡胃肠道微生物群的影响。
在四个重复的生长周期中，采用454焦磷酸测序法对16S rRNA基因的V6 - V8区域进行测序，并运用定量PCR分别检测市场龄肉鸡空肠和盲肠中的细菌微生物群和产气荚膜梭菌。
我们的结果表明，相对于BMD，维吉尼亚霉素对肉鸡胃肠道细菌群落的影响更为显著，主要表现为盲肠中粪杆菌属的显著富集以及空肠和盲肠中一个独特的乳酸杆菌种群OTU_02。在空肠或盲肠中，未观察到不同日粮间产气荚膜梭菌水平存在差异的证据。
这项工作是迄今为止对饲喂维吉尼亚霉素或BMD的肉鸡空肠和盲肠微生物群进行的分辨率最高的比较，并为通常归因于这些饲料抗生素的健康和生产性能益处中可能涉及的特定细菌OTU提供了证据。
