Abozaid Mortada A, Langlo Christopher S, Dubis Adam M, Michaelides Michel, Tarima Sergey, Carroll Joseph
Department of Ophthalmology, The Eye Institute, Medical College of Wisconsin, 925 N. 87th Street, 53226, Milwaukee, WI, USA.
Department of Ophthalmology, Sohag University, Sohag, Egypt.
Adv Exp Med Biol. 2016;854:277-83. doi: 10.1007/978-3-319-17121-0_37.
Adaptive optics scanning light ophthalmoscopy (AOSLO) allows non-invasive assessment of the cone photoreceptor mosaic. Confocal AOSLO imaging of patients with achromatopsia (ACHM) reveals an altered reflectivity of the remaining cone structure, making identification of the cells more challenging than in normal retinas. Recently, a "split-detector" AOSLO imaging method was shown to enable direct visualization of cone inner segments in patients with ACHM. Several studies have demonstrated gene replacement therapy effective in restoring cone function in animal models of ACHM and human trials have on the horizon, making the ability to reliably assess cone structure increasingly important. Here we sought to examine whether absolute estimates of cone density obtained from split-detector and confocal AOSLO images differed from one another and whether the inter- and intra-observer reliability is significantly different between these modes. These findings provide an important foundation for evaluating the role of these images as tools to assess the efficacy of future gene therapy trials.
自适应光学扫描激光检眼镜（AOSLO）可对视锥光感受器镶嵌结构进行无创评估。对色盲患者（ACHM）进行共焦AOSLO成像显示，剩余视锥结构的反射率发生了改变，这使得识别这些细胞比在正常视网膜中更具挑战性。最近，一种“分裂探测器”AOSLO成像方法被证明能够直接观察ACHM患者的视锥内节。多项研究已证明基因替代疗法在恢复ACHM动物模型中的视锥功能方面有效，并且人体试验也即将开展，因此可靠评估视锥结构的能力变得越来越重要。在此，我们试图研究从分裂探测器和共焦AOSLO图像获得的视锥密度绝对估计值是否彼此不同，以及在这些模式之间观察者间和观察者内的可靠性是否存在显著差异。这些发现为评估这些图像作为评估未来基因治疗试验疗效工具的作用提供了重要基础。
