Khodadad Nastaran, Moattari Afagh, Shamsi Shahr Abadi Mahmoud, Kadivar Mohammad Rahim, Sarvari Jamal, Tavakoli Forough, Pirbonyeh Neda, Emami Amir
Department of Bacteriology and Virology, Shiraz University of Medical Sciences, Shiraz, IR Iran.
Department of Virology, Tehran University of Medical Sciences, Tehran, IR Iran.
Jundishapur J Microbiol. 2015 Aug 29;8(8):e23690. doi: 10.5812/jjm.23690. eCollection 2015 Aug.
Oseltamivir has been used as a drug of choice for the prophylaxis and treatment of human influenza A(H1N1)pdm09 infection across the world. However, the most frequently identified oseltamivir resistant virus, influenza A(H1N1)pdm09, exhibit the H275Y substitution in NA gene.
This study aimed to determine the prevalence and phylogenetic relationships of oseltamivir resistance in influenza A(H1N1)pdm09 viruses isolated in Shiraz, Iran.
Throat swab samples were collected from 200 patients with influenza-like disease from December 2012 until February 2013. A total of 77 influenza A(H1N1)pdm09 positive strains were identified by real-time polymerase chain reaction (PCR). Oseltamivir resistance was detected using quantal assay and nested-PCR method. The NA gene sequencing was conducted to detect oseltamivir-resistant mutants and establish the phylogeny of the prevalent influenza variants.
Our results revealed that A(H1N1)pdm09 viruses present in these samples were susceptible to oseltamivir, and contained 5 site specific mutations (V13G, V106I, V241I, N248D, and N369K) in NA gene. These mutations correlated with increasing expression and enzymatic activity of NA protein in the influenza A(H1N1)pdm09 viruses, which were closely related to a main influenza A(H1N1)pdm09 cluster isolated around the world.
A(H1N1)pdm09 viruses, identified in this study in Shiraz, Iran, contained 5 site specific mutations and were susceptible to oseltamivir.
在全球范围内，奥司他韦一直被用作预防和治疗甲型H1N1流感病毒（A(H1N1)pdm09）感染的首选药物。然而，最常见的对奥司他韦耐药的病毒，即甲型H1N1流感病毒（A(H1N1)pdm09），在NA基因中出现了H275Y替换。
本研究旨在确定在伊朗设拉子分离出的甲型H1N1流感病毒（A(H1N1)pdm09）中奥司他韦耐药性的流行情况及其系统发育关系。
从2012年12月至2013年2月，收集了200例流感样疾病患者的咽拭子样本。通过实时聚合酶链反应（PCR）共鉴定出77株甲型H1N1流感病毒（A(H1N1)pdm09）阳性菌株。使用定量分析和巢式PCR方法检测奥司他韦耐药性。进行NA基因测序以检测奥司他韦耐药突变体，并建立流行的流感病毒变体的系统发育。
我们的结果显示，这些样本中存在的甲型H1N1流感病毒（A(H1N1)pdm09）对奥司他韦敏感，并且在NA基因中含有5个位点特异性突变（V13G、V106I、V241I、N248D和N369K）。这些突变与甲型H1N1流感病毒（A(H1N1)pdm09）中NA蛋白表达增加和酶活性增加相关，这些病毒与世界各地分离出的一个主要甲型H1N1流感病毒（A(H1N1)pdm09）簇密切相关。
在本研究中于伊朗设拉子鉴定出的甲型H1N1流感病毒（A(H1N1)pdm09）含有5个位点特异性突变，并且对奥司他韦敏感。
