Anwar S, Iqbal M P, Azam I, Habib A, Bhutta S, Soofi S B, Bhutta Z A
a Centre of Excellence in Women and Child Health, Aga Khan University , Stadium Road, Karachi , Pakistan.
b Department of Biological and Biomedical Sciences , Aga Khan University , Stadium Road, Karachi , Pakistan.
J Obstet Gynaecol. 2016;36(3):318-23. doi: 10.3109/01443615.2015.1050647. Epub 2015 Oct 14.
We undertook a cross-sectional study in rural Jehlum and urban Karachi to evaluate the prevalence of vitamin D deficiency in Pakistani pregnant women and neonates and to assess any association of serum 25(OH) vitamin D [25(OH)D] concentration with vitamin D binding protein (Gc) genotypes. Altogether, 390 women and 266 neonates were recruited from urban and rural sites, respectively. Serum 25(OH)D was measured by an immunoassay, while Gc genotypes were identified using polymerase chain reaction followed by restriction fragment length polymorphism or PCR-RFLP. One-way analysis of variance or ANOVA and linear regression were used for statistical analysis. In urban Karachi, 99.5% of women and 97.3% of neonates were vitamin D deficient (< 50 nmol/L), while 89% of women and 82% of neonates were deficient in rural Jehlum. Gc genotypes were not associated with serum 25(OH)D concentrations in both women and their neonates. We conclude that vitamin D deficiency is highly prevalent in Pakistani women and their neonates, and Gc genotypes are not associated with serum 25(OH)D concentrations.
我们在杰赫勒姆农村地区和卡拉奇市区开展了一项横断面研究，以评估巴基斯坦孕妇和新生儿维生素D缺乏症的患病率，并评估血清25(OH)维生素D[25(OH)D]浓度与维生素D结合蛋白（Gc）基因型之间的关联。总共分别从城市和农村地区招募了390名女性和266名新生儿。采用免疫分析法测定血清25(OH)D，同时使用聚合酶链反应随后进行限制性片段长度多态性分析或PCR-RFLP来鉴定Gc基因型。使用单因素方差分析（ANOVA）和线性回归进行统计分析。在卡拉奇市区，99.5%的女性和97.3%的新生儿维生素D缺乏（<50 nmol/L），而在杰赫勒姆农村地区，89%的女性和82%的新生儿维生素D缺乏。女性及其新生儿的Gc基因型与血清25(OH)D浓度均无关联。我们得出结论，维生素D缺乏症在巴基斯坦女性及其新生儿中非常普遍，且Gc基因型与血清25(OH)D浓度无关。
