Caldeira Patrícia Carlos, de Andrade Sousa Alexandre, de Aguiar Maria Cássia Ferreira
Department of Oral Pathology and Surgery, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, CEP: 31.270-901, Belo Horizonte, MG, Brazil.
Department of Surgery of the School of Medicine and Head and Neck Surgery, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
BMC Res Notes. 2015 Oct 14;8:569. doi: 10.1186/s13104-015-1541-x.
Recent studies have pointed towards a role of tumour-infiltrating neutrophils in cancer biology. Investigations on oral squamous cell carcinoma have indicated a possible association with clinical characteristics. This study aimed to evaluate neutrophil infiltration and the neutrophil/lymphocyte ratio in the central areas and invasive front of oral squamous cell carcinomas at different T stages, and their association with clinicopathological features and patient outcome.
Clinical information was retrieved from the charts of patients who had undergone surgical treatment. Samples of the excised tumours were subjected to immunohistochemical analysis for CD66b and CD3. Semi-quantitative analysis was performed in the intratumoural region and in the invasive front. Appropriate statistical tests were used for evaluating the data, including Kaplan-Meier survival analysis and the log-rank test. A p value of less than 0.05 was considered significant.
T3-T4 tumours presented higher CD66b infiltration in the intratumoural region and higher CD66b/CD3 ratios in the invasive front than T1-T2 lesions (p < 0.05). There was a strong inverse correlation between CD66b and CD3 in the invasive front of T3-T4 tumours (r = -0.712, p < 0.05). Comparisons of CD66b and the CD66b/CD3 ratio according to N status, tumour location, recurrence, inflammation grade, and histological grade did not reach statistical significance. Survival analysis also did not show any significant differences.
The present study showed different degrees of neutrophil infiltration between T1-T2 and T3-T4 oral cancers, with higher indexes in the advanced lesions. However, there was no association with clinicopathological features or with time to recurrence.
最近的研究表明肿瘤浸润性中性粒细胞在癌症生物学中发挥作用。对口腔鳞状细胞癌的研究表明其可能与临床特征相关。本研究旨在评估不同T分期口腔鳞状细胞癌中心区域和浸润前沿的中性粒细胞浸润及中性粒细胞/淋巴细胞比值，以及它们与临床病理特征和患者预后的关系。
从接受手术治疗的患者病历中检索临床信息。对切除肿瘤的样本进行CD66b和CD3的免疫组织化学分析。在肿瘤内区域和浸润前沿进行半定量分析。使用适当的统计检验评估数据，包括Kaplan-Meier生存分析和对数秩检验。p值小于0.05被认为具有统计学意义。
与T1-T2病变相比，T3-T4肿瘤在肿瘤内区域呈现更高的CD66b浸润，在浸润前沿呈现更高的CD66b/CD3比值（p < 0.05）。在T3-T4肿瘤的浸润前沿，CD66b与CD3之间存在强烈的负相关（r = -0.712，p < 0.05）。根据N分期、肿瘤位置、复发、炎症分级和组织学分级对CD66b和CD66b/CD3比值进行比较，未达到统计学意义。生存分析也未显示任何显著差异。
本研究显示T1-T2和T3-T4期口腔癌的中性粒细胞浸润程度不同，晚期病变的指标更高。然而，其与临床病理特征或复发时间无关。
