Schreiber Konrad, Csaba Gergely, Haslbeck Martin, Zimmer Ralf
Institut für Informatik, Ludwig-Maximilians-Universität München, München, Germany.
Department Chemie, Technische Universität München, Garching, Germany.
PLoS One. 2015 Oct 15;10(10):e0140487. doi: 10.1371/journal.pone.0140487. eCollection 2015.
mRNA splicing is required in about 4% of protein coding genes in Saccharomyces cerevisiae. The gene structure of those genes is simple, generally comprising two exons and one intron. In order to characterize the impact of alternative splicing on the S. cerevisiae transcriptome, we perform a systematic analysis of mRNA sequencing data. We find evidence of a pervasive use of alternative splice sites and detect several novel introns both within and outside protein coding regions. We also find a predominance of alternative splicing on the 3' side of introns, a finding which is consistent with existing knowledge on conservation of exon-intron boundaries in S. cerevisiae. Some of the alternatively spliced transcripts allow for a translation into different protein products.
在酿酒酵母中，约4%的蛋白质编码基因需要mRNA剪接。这些基因的结构简单，通常由两个外显子和一个内含子组成。为了表征可变剪接对酿酒酵母转录组的影响，我们对mRNA测序数据进行了系统分析。我们发现了广泛使用可变剪接位点的证据，并在蛋白质编码区域内外检测到了几个新的内含子。我们还发现内含子3'端存在可变剪接优势，这一发现与酿酒酵母中外显子-内含子边界保守性的现有知识一致。一些可变剪接转录本可以翻译为不同的蛋白质产物。
