Lin Chien-Hong, Cho Hsien-Lung, Yeh Yi-Hsing, Yang Ming-Chien
Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
Department of Materials Science and Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:735-43. doi: 10.1016/j.colsurfb.2015.10.006. Epub 2015 Oct 27.
The surface wettability and anti-protein adsorption of a silicone-based hydrogel that was synthesized by a block copolymer of polydimethylsiloxane (PDMS) and poly (ethylene glycol) methacrylate (PEGMA) was improved via polyelectrolyte multilayer (PEM) immobilization. Polysaccharide PEMs of chitosan (CS, as a positive-charged agent) and hyaluronic acid (HA, as a negative-charged and anti-adhesive agent) were successfully assembled on the PDMS-PU-PEGMA silicone hydrogel in a layer-by-layer (LBL) self-assembly manner. Atomic force microscopy (AFM) and dyeing data verified the progressive buildup of the PEM silicone hydrogel. The results showed that the contact angle of the silicone hydrogel decreased with an increase in the number of PEM grafting layers. Furthermore, after immobilizing five layers of CS/HA, the protein adsorption decreased from 78 ± 11 to 26 ± 4 μg/cm(2) for HSA and from 55 ± 10 to 20 ± 4 μg/cm(2) for lysozymes. This indicates that CS/HA PEM-immobilized silicone hydrogels can resist protein adsorption. Furthermore, these hydrogels were non-cytotoxic according to an in vitro L929 fibroblast assay. Overall, the results demonstrated that the modified silicone hydrogels exhibited hydrophilicity and anti-protein adsorption, as well as relatively high oxygen permeability and optical transparency. Therefore, they would be applicable as a contact lens material.
通过聚电解质多层（PEM）固定化，改善了由聚二甲基硅氧烷（PDMS）和聚（甲基丙烯酸乙二醇酯）（PEGMA）的嵌段共聚物合成的硅基水凝胶的表面润湿性和抗蛋白质吸附性能。壳聚糖（CS，作为带正电荷的试剂）和透明质酸（HA，作为带负电荷的抗粘附试剂）的多糖PEM以逐层（LBL）自组装方式成功组装在PDMS-PU-PEGMA硅水凝胶上。原子力显微镜（AFM）和染色数据验证了PEM硅水凝胶的逐步形成。结果表明，硅水凝胶的接触角随着PEM接枝层数的增加而减小。此外，固定五层CS/HA后，人血清白蛋白（HSA）的蛋白质吸附量从78±11μg/cm²降至26±4μg/cm²，溶菌酶的蛋白质吸附量从55±10μg/cm²降至20±4μg/cm²。这表明CS/HA PEM固定化的硅水凝胶能够抵抗蛋白质吸附。此外，根据体外L929成纤维细胞试验，这些水凝胶无细胞毒性。总体而言，结果表明改性硅水凝胶具有亲水性和抗蛋白质吸附性，以及相对较高的透氧性和光学透明度。因此，它们可作为隐形眼镜材料应用。
