Tsuda Soichiro, Jaffery Hussain, Doran David, Hezwani Mohammad, Robbins Phillip J, Yoshida Mari, Cronin Leroy
WestCHEM, School of Chemistry, University of Glasgow, Glasgow, United Kingdom.
Institute of Molecular Cell and Systems Biology, University of Glasgow, Glasgow, United Kingdom.
PLoS One. 2015 Nov 11;10(11):e0141640. doi: 10.1371/journal.pone.0141640. eCollection 2015.
Three dimensional (3D) printing is actively sought after in recent years as a promising novel technology to construct complex objects, which scope spans from nano- to over millimeter scale. Previously we utilized Fused deposition modeling (FDM)-based 3D printer to construct complex 3D chemical fluidic systems, and here we demonstrate the construction of 3D milli-fluidic structures for programmable liquid handling and control of biological samples. Basic fluidic operation devices, such as water-in-oil (W/O) droplet generators for producing compartmentalized mono-disperse droplets, sensor-integrated chamber for online monitoring of cellular growth, are presented. In addition, chemical surface treatment techniques are used to construct valve-based flow selector for liquid flow control and inter-connectable modular devices for networking fluidic parts. As such this work paves the way for complex operations, such as mixing, flow control, and monitoring of reaction / cell culture progress can be carried out by constructing both passive and active components in 3D printed structures, which designs can be shared online so that anyone with 3D printers can reproduce them by themselves.
近年来，三维（3D）打印作为一种有前途的新型技术，被积极地应用于构建复杂物体，其范围涵盖从纳米到毫米以上的尺度。此前，我们利用基于熔融沉积建模（FDM）的3D打印机构建了复杂的3D化学流体系统，在此我们展示了用于可编程液体处理和生物样品控制的3D微流体结构的构建。展示了基本的流体操作装置，如用于产生分隔的单分散液滴的油包水（W/O）液滴发生器、用于在线监测细胞生长的集成传感器腔室。此外，化学表面处理技术用于构建基于阀门的流量选择器以控制液体流动，以及用于流体部件联网的可相互连接的模块化装置。因此，这项工作为复杂操作铺平了道路，例如通过在3D打印结构中构建被动和主动组件，可以进行混合、流量控制以及反应/细胞培养进程的监测，这些设计可以在线共享，以便任何拥有3D打印机的人都能自行复制。
