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傅里叶显微镜成像配置与目标的对比分析

Comparative analysis of imaging configurations and objectives for Fourier microscopy.

作者信息

Kurvits Jonathan A, Jiang Mingming, Zia Rashid

出版信息

J Opt Soc Am A Opt Image Sci Vis. 2015 Nov 1;32(11):2082-92. doi: 10.1364/JOSAA.32.002082.

Abstract

Fourier microscopy is becoming an increasingly important tool for the analysis of optical nanostructures and quantum emitters. However, achieving quantitative Fourier space measurements requires a thorough understanding of the impact of aberrations introduced by optical microscopes that have been optimized for conventional real-space imaging. Here we present a detailed framework for analyzing the performance of microscope objectives for several common Fourier imaging configurations. To this end, we model objectives from Nikon, Olympus, and Zeiss using parameters that were inferred from patent literature and confirmed, where possible, by physical disassembly. We then examine the aberrations most relevant to Fourier microscopy, including the alignment tolerances of apodization factors for different objective classes, the effect of magnification on the modulation transfer function, and vignetting-induced reductions of the effective numerical aperture for wide-field measurements. Based on this analysis, we identify an optimal objective class and imaging configuration for Fourier microscopy. In addition, the Zemax files for the objectives and setups used in this analysis have been made publicly available as a resource for future studies.

摘要

傅里叶显微镜正日益成为分析光学纳米结构和量子发射器的重要工具。然而,要实现定量的傅里叶空间测量,需要深入了解为传统实空间成像而优化的光学显微镜所引入的像差的影响。在此,我们提出了一个详细的框架,用于分析几种常见傅里叶成像配置下显微镜物镜的性能。为此,我们使用从专利文献中推断并在可能情况下通过物理拆解确认的参数,对尼康、奥林巴斯和蔡司的物镜进行建模。然后,我们研究与傅里叶显微镜最相关的像差,包括不同物镜类别切趾因子的对准公差、放大倍数对调制传递函数的影响,以及宽视场测量中渐晕导致的有效数值孔径减小。基于此分析,我们确定了傅里叶显微镜的最佳物镜类别和成像配置。此外,本分析中使用的物镜和设置的Zemax文件已公开提供,作为未来研究的资源。

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