Federici Antoine, Dubois Arnaud
Opt Lett. 2015 Nov 15;40(22):5347-50. doi: 10.1364/OL.40.005347.
Full-field optical coherence microscopy (FF-OCM) with isotropic spatial resolution of 0.5 μm (in water), at 700 nm center wavelength, is reported. A theoretical study of the FF-OCM axial response is carried out for maximizing the axial resolution of the system, considering the effect of optical dispersion. The lateral resolution is optimized by using water-immersion microscope objectives with a numerical aperture of 1.2. This ultrahigh-resolution FF-OCM system is applied to animal and human skin tissue imaging, revealing ultra-fine in-depth structures at the sub-cellular level.
据报道，全视野光学相干显微镜（FF-OCM）在中心波长700nm时，在水中具有0.5μm的各向同性空间分辨率。考虑到光学色散的影响，对FF-OCM轴向响应进行了理论研究，以最大化系统的轴向分辨率。通过使用数值孔径为1.2的水浸显微镜物镜来优化横向分辨率。这种超高分辨率的FF-OCM系统应用于动物和人体皮肤组织成像，揭示了亚细胞水平的超精细深度结构。
