Zhang Fengbo, Li Zhiwei, La Xiaolin, Ma Xiumin, Zhang Yaoxin, Ji Ping, Jiang Min, Hu Jinwei, Zhang Zhaoxia, Lu Xiaobo, Ding Jianbing
Department of Clinical Laboratory, The First Affiliated Hospital of Xinjiang Medical University Urumqi 830054, Xinjiang, China ; Department of Immunology, School of Preclinical Medicine, Xinjiang Medical University Urumqi 830011, Xinjiang, China.
Department of Clinical Laboratory, Xinjiang People's Hospital Urumqi 830000, Xinjiang, China.
Int J Clin Exp Med. 2015 Sep 15;8(9):15716-23. eCollection 2015.
This study is to characterize and identify the human Brucella strains in Xinjiang, China with multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) scheme.
Brucella strains were isolated and cultured from 62 brucellosis patients. The bacteria strains were subjected to the oxidase, catalase, rapid urease, and nitrate reduction tests, and the species identification was performed using the VITEK-2 Compact system. These Brucella strains were further identified and characterized using the 16 VNTR loci in a MLVA-16 methodology.
Twelve Brucella strains had been identified out of 62 patients, which were all recognized as Brucella melitensis (B. melitensis) according to the results from the VITEK-2 Compact system. Based on panel 1 (MLVA-8), these 12 Brucella isolates were clustered into three known genotypes and two new genotypes, in which 7 strains were clustered into genotype 45 (1-5-3-12-2-2-3-2), 1 strain was classified as genotype 42 (1-5-3-13-2-2-3-2), 1 stain was with genotype 62 (1-3-3-13-2-2-3-2), and the other 3 trains revealed two new genotypes, i.e., (1-5-3-12-2-3-3-2) and (1-5-3-11-2-3-3-2). Using panel 2A+2B (MLVA-16), we found that no genotypes of these strains were identical to the known genotypes, generally with differences in 2-4 loci. However, three strains shared the same genotype.
Brucella strains in 62 brucellosis patients from Xinjiang are all identified as B. melitensis. Based on MLVA-8, two new genotypes have been discovered. These findings might contribute to the understanding of the pathogenesis and epidemiology of brucellosis in Xinjiang, China.
本研究旨在通过多位点可变数目串联重复序列（VNTR）分析（MLVA）方案对中国新疆的人布鲁氏菌菌株进行特征分析和鉴定。
从62例布鲁氏菌病患者中分离培养布鲁氏菌菌株。对菌株进行氧化酶、过氧化氢酶、快速尿素酶和硝酸盐还原试验，并使用VITEK-2 Compact系统进行菌种鉴定。使用MLVA-16方法中的16个VNTR位点对这些布鲁氏菌菌株进行进一步鉴定和特征分析。
62例患者中共鉴定出12株布鲁氏菌菌株，根据VITEK-2 Compact系统的结果，这些菌株均被鉴定为羊种布鲁氏菌（B. melitensis）。基于第一组（MLVA-8），这12株布鲁氏菌分离株被聚类为3种已知基因型和2种新基因型，其中7株聚类为基因型45（1-5-3-12-2-2-3-2），1株分类为基因型42（1-5-3-13-2-2-3-2），1株为基因型62（1-3-3-13-2-2-3-2），另外3株显示出两种新基因型，即（1-5-3-12-2-3-3-2）和（1-5-3-11-2-3-3-2）。使用第二组A+2B（MLVA-16），我们发现这些菌株的基因型与已知基因型均不相同，通常在2-4个位点存在差异。然而，有3株菌株具有相同的基因型。
新疆62例布鲁氏菌病患者中的布鲁氏菌菌株均被鉴定为羊种布鲁氏菌。基于MLVA-8，发现了两种新基因型。这些发现可能有助于了解中国新疆布鲁氏菌病的发病机制和流行病学。
