State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Biomed Res Int. 2018 May 15;2018:2186027. doi: 10.1155/2018/2186027. eCollection 2018.
Animal brucellosis is a reemerging disease in China, particular in northwest China. The species (even genus) are highly conserved; therefore the use of Multilocus sequencing typing (MLST: based on conserved housekeeping loci) is more suitable for discrimination at species or biovar level on . In this study, MLST was used to analyze the characterization of from sheep and yaks during 2015 and 2016. All 66 isolates were collected from northwest China, including Inner Mongolia, Xinjiang, Qinghai, and Gansu provinces. Isolates were cultured on agar medium and identified by MLST. MLST identified five ST types: ST8 ( = 55), ST7 ( = 2), ST3 ( = 5), ST1 ( = 2), and ST14 ( = 2). This analysis revealed that isolates exhibited high single genotypes (ST8) in the most northwest China. MLST of isolates provides helpful information on understanding genetic characterization of in northwest China.
动物布鲁氏菌病在中国重新出现，特别是在中国西北地区。这些物种（甚至属）高度保守；因此，多基因座序列分型（MLST：基于保守的管家基因座）更适合于在物种或生物型水平上进行区分。在这项研究中，我们使用 MLST 来分析 2015 年和 2016 年从绵羊和牦牛中分离的布鲁氏菌的特征。所有 66 株分离株均来自中国西北地区，包括内蒙古、新疆、青海和甘肃。分离株在 5%羊血琼脂培养基上培养，并通过 MLST 进行鉴定。MLST 鉴定出 5 种 ST 型：ST8（=55）、ST7（=2）、ST3（=5）、ST1（=2）和 ST14（=2）。这项分析表明，在中国最西北的地区，分离株表现出高度单一的基因型（ST8）。分离株的 MLST 为了解中国西北地区 的遗传特征提供了有价值的信息。
