Genetic Characterization of Animal Isolates from Northwest Region in China.

Animal brucellosis is a reemerging disease in China, particular in northwest China. The species (even genus) are highly conserved; therefore the use of Multilocus sequencing typing (MLST: based on conserved housekeeping loci) is more suitable for discrimination at species or biovar level on . In this study, MLST was used to analyze the characterization of from sheep and yaks during 2015 and 2016. All 66 isolates were collected from northwest China, including Inner Mongolia, Xinjiang, Qinghai, and Gansu provinces. Isolates were cultured on agar medium and identified by MLST. MLST identified five ST types: ST8 ( = 55), ST7 ( = 2), ST3 ( = 5), ST1 ( = 2), and ST14 ( = 2). This analysis revealed that isolates exhibited high single genotypes (ST8) in the most northwest China. MLST of isolates provides helpful information on understanding genetic characterization of in northwest China.