Goh Jorming, Ladiges Warren
Defence Medical and Environmental Research Institute, DSO National Laboratories, Republic of Singapore.
Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Republic of Singapore.
Curr Protoc Mouse Biol. 2015 Dec 2;5(4):283-290. doi: 10.1002/9780470942390.mo140295.
Voluntary wheel running in the mouse is used to assess physical performance and endurance and to model exercise training as a way to enhance health. Wheel running is a voluntary activity in contrast to other experimental exercise models in mice, which rely on aversive stimuli to force active movement. This protocol consists of allowing mice to run freely on the open surface of a slanted, plastic saucer-shaped wheel placed inside a standard mouse cage. Rotations are electronically transmitted to a USB hub so that frequency and rate of running can be captured via a software program for data storage and analysis for variable time periods. Mice are individually housed so that accurate recordings can be made for each animal. Factors such as mouse strain, gender, age, and individual motivation, which affect running activity, must be considered in the design of experiments using voluntary wheel running.
小鼠自愿进行轮转运动可用于评估身体机能和耐力，并将运动训练作为促进健康的一种方式进行模拟。与小鼠的其他实验性运动模型不同，轮转运动是一种自愿活动，其他模型依靠厌恶刺激来迫使小鼠进行主动运动。该方案包括让小鼠在置于标准小鼠笼内的倾斜塑料碟形轮子的开放表面上自由奔跑。转动通过电子方式传输到USB集线器，以便可以通过软件程序捕获奔跑频率和速率，用于在不同时间段进行数据存储和分析。小鼠单独饲养，以便能够对每只动物进行准确记录。在使用自愿轮转运动的实验设计中，必须考虑影响奔跑活动的因素，如小鼠品系、性别、年龄和个体动机。
