Jorjão Adeline Lacerda, de Oliveira Felipe Eduardo, Leão Mariella Vieira Pereira, Carvalho Cláudio Antonio Talge, Jorge Antonio Olavo Cardoso, de Oliveira Luciane Dias
Department of Biosciences and Oral Diagnosis, Laboratory of Microbiology and Immunology, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo, 777 Jardim São Dimas, 12245-000 São José dos Campos, SP, Brazil.
Bioscience Basic Institute, University of Taubaté, Street Expedicionário Ernesto Pereira 3, 12020-130 Taubaté, SP, Brazil.
ScientificWorldJournal. 2015;2015:716749. doi: 10.1155/2015/716749. Epub 2015 Nov 16.
This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0.05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0.05) but not IL-6 (P > 0.05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0.05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.
本研究旨在评估鼠李糖乳杆菌及其产物诱导小鼠巨噬细胞（RAW 264.7）合成细胞因子（TNF-α、IL-1β、IL-4、IL-6、IL-10和IL-12）的能力。使用了三种微生物制剂：活鼠李糖乳杆菌（LLR）悬液、热灭活鼠李糖乳杆菌（HKLR）悬液和热灭活鼠李糖乳杆菌悬液的上清液（SHKLR），将它们与巨噬细胞在37°C、5% CO₂条件下培养2小时30分钟。之后，细胞再培养16小时。通过酶联免疫吸附测定法（ELISA）对上清液进行细胞因子定量分析。将结果与脂多糖（LPS）诱导的合成结果进行比较，并使用方差分析（ANOVA）和5%的Tukey检验进行分析。LLR组和HKLR组能够显著增加TNF-α、IL-6和IL-10的产生（P < 0.05）。SHKLR也显著增加了TNF-α和IL-10的产生（P < 0.05），但未增加IL-6的产生（P > 0.05）。所有鼠李糖乳杆菌悬液均未产生可检测水平的IL-1β或显著水平的IL-4和IL-12（P > 0.05）。总之，活的和热灭活的鼠李糖乳杆菌悬液能够诱导具有促炎（TNF-α和IL-6）或调节（IL-10）功能的不同细胞因子的合成，这表明鼠李糖乳杆菌ATCC 7469菌株在调节或刺激免疫反应中发挥作用。
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