Hu Baoying, Yan Xia, Liu Fang, Zhu Changlai, Zhou Huiling, Chen Yuyan, Liu Jinxia, Gu Xingxing, Ni Runzhou, Zhang Tianyi
Basic Medic Research Centre, Medical College, Nantong University, Nantong, 226001, People's Republic of China.
Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu, People's Republic of China.
Pathol Oncol Res. 2016 Jul;22(3):555-65. doi: 10.1007/s12253-015-0038-1. Epub 2015 Dec 29.
Human hepatocellular carcinoma (HCC) is one of the most common malignant cancers, whose molecular mechanisms is remains largely. PTPN9 has recently been reported to play a critical role in breast cancer development. However, the role of PTPN9 in human HCC remains elusive. The present study aimed at investigating the potential role of PTPN9 in HCC. Western blot and immunohistochemistry were used to examine the expression of PTPN9 protein in HCC and adjacent non-tumorous tissues in 45 patients. Furthermore, Cell Counting Kit-8, flow cytometry and RNA interference experiments were performed to analyze the role of PTPN9 in the regulation of HCC cell proliferation. We showed that the expression level of PTPN9 was significantly reduced in HCC, compared with adjacent non-tumorous tissues. PTPN9 expression was inversely associated with Tumor size (P = 0.014), serum AFP level (P = 0.004) and Ki-67 expression. Low expression of PTPN9 predicted poor survival in HCC patients. Moreover, PTPN9 interference assay that PTPN9 inhibited cell proliferation in HepG2 cells. Cell apoptosis assay revealed that, silencing of PTPN9 expression significantly reduced cell apoptosis, compared with control ShRNA treatment group. Our results suggested that PTPN9 expression was down-regulated in HCC tumor tissues, and reduced PTPN9 expression was associated with worsened overall survival in HCC patients. Depletion of PTPN9 inhibits the apoptosis and promotes the proliferation of HCC cells.
人类肝细胞癌（HCC）是最常见的恶性肿瘤之一，其分子机制在很大程度上仍不清楚。最近有报道称PTPN9在乳腺癌发展中起关键作用。然而，PTPN9在人类HCC中的作用仍然难以捉摸。本研究旨在探讨PTPN9在HCC中的潜在作用。采用蛋白质免疫印迹法和免疫组织化学法检测45例HCC患者及其癌旁非肿瘤组织中PTPN9蛋白的表达。此外，进行细胞计数试剂盒-8、流式细胞术和RNA干扰实验，以分析PTPN9在调节HCC细胞增殖中的作用。我们发现，与癌旁非肿瘤组织相比，HCC中PTPN9的表达水平显著降低。PTPN9表达与肿瘤大小（P = 0.014）、血清甲胎蛋白水平（P = 0.004）和Ki-67表达呈负相关。PTPN9低表达预示着HCC患者的生存预后较差。此外，PTPN9干扰试验表明PTPN9抑制HepG2细胞的增殖。细胞凋亡试验显示，与对照短发夹RNA处理组相比，PTPN9表达沉默显著降低细胞凋亡。我们的结果表明，HCC肿瘤组织中PTPN9表达下调，PTPN9表达降低与HCC患者总体生存率恶化相关。PTPN9的缺失抑制HCC细胞的凋亡并促进其增殖。
