人流感病毒血凝素基因抗原决定簇在大肠杆菌中的表达。
Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli.
作者信息
Davis A R, Nayak D P, Ueda M, Hiti A L, Dowbenko D, Kleid D G
出版信息
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5376-80. doi: 10.1073/pnas.78.9.5376.
Antigenic determinants of influenza virus hemagglutinin were expressed in Escherichia coli. DNA coding for presequences of hemagglutinin were removed and an ATG codon was placed before DNA coding for mature hemagglutinin. A number of expression plasmids were constructed in which various segments of this reconstructed hemagglutinin DNA were fused to DNA coding for bacterial beta-galactosidase. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.
流感病毒血凝素的抗原决定簇在大肠杆菌中表达。去除编码血凝素前导序列的DNA，并在编码成熟血凝素的DNA之前放置一个ATG密码子。构建了许多表达质粒，其中这种重建的血凝素DNA的各个片段与编码细菌β-半乳糖苷酶的DNA融合。融合蛋白表现出与抗病毒抗体的特异性结合。这种结合可被过量的病毒血凝素竞争性抑制，表明这些融合蛋白含有血凝素的抗原决定簇。
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