Department of Biology, Huck Institutes of Life Sciences, The Pennsylvania State University , University Park, Pennsylvania 16802.
Institute for Stem Cell Biology and Regenerative Medicine , Stanford, California 94305.
eNeuro. 2015 Nov 9;2(5). doi: 10.1523/ENEURO.0106-15.2015. eCollection 2015 Sep-Oct.
Cell reprogramming technologies have enabled the generation of various specific cell types including neurons from readily accessible patient cells, such as skin fibroblasts, providing an intriguing novel cell source for autologous cell transplantation. However, cell transplantation faces several difficult hurdles such as cell production and purification, long-term survival, and functional integration after transplantation. Recently, in vivo reprogramming, which makes use of endogenous cells for regeneration purpose, emerged as a new approach to circumvent cell transplantation. There has been evidence for in vivo reprogramming in the mouse pancreas, heart, and brain and spinal cord with various degrees of success. This mini review summarizes the latest developments presented in the first symposium on in vivo reprogramming glial cells into functional neurons in the brain and spinal cord, held at the 2014 annual meeting of the Society for Neuroscience in Washington, DC.
细胞重编程技术使人们能够从易于获得的患者细胞（如皮肤成纤维细胞）中产生各种特定的细胞类型，包括神经元，为自体细胞移植提供了一种有吸引力的新型细胞来源。然而，细胞移植面临着许多困难的障碍，如细胞的生产和纯化、长期存活以及移植后的功能整合。最近，体内重编程作为一种避开细胞移植的新方法出现了，它利用内源性细胞来实现再生的目的。在小鼠的胰腺、心脏和大脑及脊髓中，已经有体内重编程的证据，并且取得了不同程度的成功。这篇迷你综述总结了 2014 年在华盛顿特区举行的神经科学学会年会上举行的第一届关于在大脑和脊髓中将神经胶质细胞重编程为功能性神经元的体内重编程研讨会中提出的最新进展。
