Dipartimento di Agraria, Università di Napoli Federico II, Via Università 100, 80055 Portici (NA), Italy.
Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Hezar-Jerib Ave., 81746 73461 Isfahan, Iran.
Mar Drugs. 2019 Feb 1;17(2):86. doi: 10.3390/md17020086.
The saponin composition of a specimen of black sea cucumber, collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy. The saponin composition of turned out to be more complex than previously reported. The most abundant saponins in the extract (⁻) were isolated and characterized by 1D- and 2D-NMR experiments. Compound was identified as a new triterpene glycoside saponin, holothurin A5. The side chain of the new saponin , unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions. The cytotoxic activity of compounds ⁻, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC values ranging from 1.2 to 2.5 µg/mL.
对在波斯湾采集的黑海参标本的皂苷成分进行了研究，采用了包括 LC-MS/MS、分子网络、纯化合物分离和 NMR 光谱在内的综合方法。结果表明，该海参的皂苷成分比以前报道的更为复杂。提取物中（⁻）含量最丰富的皂苷被分离并通过 1D 和 2D-NMR 实验进行了表征。化合物 被鉴定为一种新的三萜糖苷皂苷，海冬青 A5。该新皂苷的侧链在三萜糖苷中是前所未有的，其特征是具有亲电烯酮官能团，在中性条件下可以缓慢地与水或甲醇加成。在所评估的人宫颈癌细胞系 HeLa 上，化合物 ⁻ 的细胞毒性活性非常显著，IC 值范围为 1.2 至 2.5 µg/mL。
