Wang Yachao, Jiang Li, Li Yuanfeng, Luo Xuegang, He Jian
School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
Engineering Research Center of Biomass Materials, Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan, 621010, China.
Biol Trace Elem Res. 2016 Aug;172(2):481-487. doi: 10.1007/s12011-015-0596-9. Epub 2016 Jan 6.
Excessive selenium (Se) intake is harmful for animals and humans. The aim of the present study was to examine the effect of long-term excessive Se supplementation on oxidative stress and endoplasmic reticulum (ER) stress-related injuries in chicken spleen. A total of 180 1-day-old chickens were randomly divided into four groups with different Se dietary contents (0.2 mg/kg Se, 5 mg/kg Se, 10 mg/kg Se, or 15 mg/kg Se) for 45 days. Then, the levels of antioxidative enzymes, GPx, SOD, and MDA as well as the expression levels of GRP78, ARF6, caspase 3, caspase 12, and Bcl 2 in the spleen were determined at days 15, 30, and 45, respectively. The results showed that excessive Se treatment decreased the activities of GPx and SOD (P < 0.05) but increased the levels of MDA (P < 0.05) in a dose- and time-dependent manner. In addition, the ER stress genes GRP78 and ATF6 were highly expressed (P < 0.05), and the apoptosis genes caspase 3 and caspase 12 were increased, but Bcl 2 was decreased by Se treatment (P < 0.05). Correlation analysis showed that there was a high correlation between these biomarkers, which indicated that ER stress and ER stress-related apoptosis were correlated with oxidative stress. These results showed the important role of oxidative stress and ER stress in Se-related immune injuries in chicken.
过量的硒（Se）摄入对动物和人类有害。本研究的目的是探讨长期过量补充硒对鸡脾脏氧化应激和内质网（ER）应激相关损伤的影响。将180只1日龄雏鸡随机分为四组，分别给予不同硒含量（0.2 mg/kg硒、5 mg/kg硒、10 mg/kg硒或15 mg/kg硒）的日粮，持续45天。然后，分别在第15天、第30天和第45天测定脾脏中抗氧化酶、谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和丙二醛（MDA）的水平，以及葡萄糖调节蛋白78（GRP78）、ADP核糖基化因子6（ARF6）、半胱天冬酶3（caspase 3）、半胱天冬酶12（caspase 12）和Bcl-2的表达水平。结果表明，过量硒处理以剂量和时间依赖性方式降低了GPx和SOD的活性（P<0.05），但增加了MDA的水平（P<0.05）。此外，硒处理使内质网应激基因GRP78和活化转录因子6（ATF6）高表达（P<0.05），凋亡基因caspase 3和caspase 12增加，但Bcl-2减少（P<0.05）。相关性分析表明，这些生物标志物之间存在高度相关性，这表明内质网应激和内质网应激相关凋亡与氧化应激相关。这些结果表明氧化应激和内质网应激在鸡硒相关免疫损伤中起重要作用。
