Hirokawa Yasutaka, Maki Yuki, Tatsuke Tsuneyuki, Hanai Taizo
Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Laboratory for Bioinformatics, Graduate School of Systems Biosciences, Kyushu University, 804 Westwing, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Metab Eng. 2016 Mar;34:97-103. doi: 10.1016/j.ymben.2015.12.008. Epub 2016 Jan 6.
Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79±0.23 mM; 288±17.7 mg/L) and glycerol (12.62±1.55 mM; 1.16±0.14 g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study.
利用光能直接将二氧化碳转化为化学品是实现可持续未来的一个有吸引力的选择。通过构建合成代谢途径的工程化聚球藻PCC 7942实现了直接从二氧化碳生产1,3 - 丙二醇（1,3 - PDO）。催化甘油以依赖辅酶B12的方式转化为3 - 羟基丙醛的甘油脱水酶在不添加维生素B12的聚球藻PCC 7942中起作用，这表明内在的假维生素B12可作为辅酶B12的替代物。在本研究的优化条件下培养14天后，1,3 - PDO（3.79±0.23 mM；288±17.7 mg/L）和1,3 - PDO的前体甘油（12.62±1.55 mM；1.16±0.14 g/L）达到了最高滴度。
