Drago Lorenzo, De Grandi Roberta, Altomare Gianfranco, Pigatto Paolo, Rossi Oliviero, Toscano Marco
Clinical Chemistry and Microbiology Laboratory, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20164 Milan, Italy ; Medical Technical Sciences Laboratory, Department of Biomedical Science for Health, University of Milan, Via Mangiagalli 31, 20133 Milan, Italy.
Medical Technical Sciences Laboratory, Department of Biomedical Science for Health, University of Milan, Via Mangiagalli 31, 20133 Milan, Italy.
Clin Mol Allergy. 2016 Jan 25;14:2. doi: 10.1186/s12948-016-0038-z. eCollection 2016.
Psoriasis and atopic dermatitis (AD) are chronic inflammatory skin diseases, which negatively influence the quality of life. In the last years, several evidences highlighted the pivotal role of skin bacteria in worsening the symptomatology of AD and psoriasis. In the present study we evaluated the skin microbiota composition in accurately selected subjects affected by (AD) and psoriasis.
Three first cousins were chosen for the study according to strict selection of criteria. One subject was affected by moderate AD, one had psoriasis and the last one was included as healthy control. Two lesional skin samples and two non-lesional skin samples (for AD and psoriatic subjects) from an area of 2 cm(2) behind the left ear were withdrawn by mean of a curette. For the healthy control, two skin samples from an area of 2 cm(2) behind the left ear were withdrawn by mean of a curette. DNA was extracted and sequencing was completed on the Ion Torrent PGM platform. Culturing of Staphylococcus aureus from skin samples was also performed.
The psoriatic subject showed a decrease in Firmicutes abundance and an increase in Proteobacteria abundance. Moreover, an increase in Streptococcaceae, Rhodobacteraceae, Campylobacteraceae and Moraxellaceae has been observed in psoriatic subject, if compared with AD individual and control. Finally, AD individual showed a larger abundance of S. aureus than psoriatic and healthy subjects. Moreover, the microbiota composition of non-lesional skin samples belonging to AD and psoriatic individuals was very similar to the bacterial composition of skin sample belonging to the healthy control.
Significant differences between the skin microbiota of psoriatic individual and healthy and AD subjects were observed.
银屑病和特应性皮炎（AD）是慢性炎症性皮肤病，对生活质量有负面影响。近年来，多项证据凸显了皮肤细菌在加重AD和银屑病症状方面的关键作用。在本研究中，我们评估了精准挑选的AD和银屑病患者的皮肤微生物群组成。
根据严格的选择标准挑选了三位表亲参与研究。一名受试者患有中度AD，一名患有银屑病，最后一名作为健康对照。通过刮匙从左耳后方2平方厘米区域采集两名AD和银屑病受试者的两份皮损皮肤样本和两份非皮损皮肤样本。对于健康对照，通过刮匙从左耳后方2平方厘米区域采集两份皮肤样本。提取DNA并在Ion Torrent PGM平台上完成测序。还对皮肤样本进行了金黄色葡萄球菌培养。
银屑病受试者显示厚壁菌门丰度降低，变形菌门丰度增加。此外，与AD个体和对照相比，银屑病受试者中链球菌科、红杆菌科、弯曲菌科和莫拉菌科有所增加。最后，AD个体的金黄色葡萄球菌丰度高于银屑病和健康受试者。此外，AD和银屑病个体的非皮损皮肤样本的微生物群组成与健康对照的皮肤样本细菌组成非常相似。
观察到银屑病个体与健康和AD受试者的皮肤微生物群存在显著差异。
