Huss Monika K, Chum Helen H, Chang Angela G, Jampachairsi Katechan, Pacharinsak Cholawat
Department of Comparative Medicine, Stanford University, Stanford, California;, Email:
Department of Laboratory Animal Resources, University of California Berkeley, Berkeley, California.
J Am Assoc Lab Anim Sci. 2016 Jan;55(1):83-8.
Information regarding effective anesthetic regimens for neonatal rat pups is limited. Here we investigated whether isoflurane or sevoflurane anesthesia maintains physiologic parameters more consistently than does hypothermia anesthesia in neonatal rat pups. Rat pups (age, 4 d) were randomly assigned to receive isoflurane, sevoflurane, or hypothermia. Physiologic parameters monitored at 1, 5, 10, and 15 min included heart rate (HR), respiratory rate (RR), and oxygen saturation (%SpO2). Other parameters evaluated were loss and return of righting reflex, paw withdrawal reflex, and maternal acceptance. Corticosterone and glucose were sampled at 20 min and 24 h after anesthesia induction. Once a surgical plane of anesthesia was achieved, a skin incision was made on the right lateral thigh. After the procedure, all pups were accepted and cared for by their dam. Isoflurane- and sevoflurane-treated pups maintained higher HR, RR, %SpO2, and glucose levels than did hypothermia-treated pups. For both the isoflurane and sevoflurane groups, HR and RR were significantly lower at 10 and 15 min after anesthesia than at 1 min. Compared with hypothermia, isoflurane and sevoflurane anesthesia provided shorter times to loss of and return of the righting reflex. Although corticosterone did not differ among the groups, glucose levels were higher at 20 min after anesthesia induction than at 24 h in all anesthetic groups. We conclude that both isoflurane and sevoflurane anesthesia maintain physiologic parameters (HR, RR, %SpO2) more consistently than does hypothermia anesthesia in 4-d-old rat pups.
关于新生大鼠幼崽有效麻醉方案的信息有限。在此，我们研究了异氟烷或七氟烷麻醉在新生大鼠幼崽中是否比低温麻醉更能持续维持生理参数。将大鼠幼崽（4日龄）随机分为接受异氟烷、七氟烷或低温处理组。在1、5、10和15分钟时监测的生理参数包括心率（HR）、呼吸频率（RR）和血氧饱和度（%SpO2）。评估的其他参数为翻正反射的消失和恢复、爪部退缩反射以及母鼠接受情况。在麻醉诱导后20分钟和24小时采集皮质酮和葡萄糖样本。一旦达到手术麻醉平面，在右大腿外侧做一个皮肤切口。手术后，所有幼崽均被母鼠接纳并得到照料。与低温处理的幼崽相比，接受异氟烷和七氟烷处理的幼崽维持了更高的HR、RR、%SpO2和葡萄糖水平。对于异氟烷和七氟烷组，麻醉后10和15分钟时的HR和RR显著低于1分钟时。与低温相比，异氟烷和七氟烷麻醉导致翻正反射消失和恢复的时间更短。尽管各组之间皮质酮无差异，但所有麻醉组在麻醉诱导后20分钟时的葡萄糖水平高于24小时时。我们得出结论，在4日龄大鼠幼崽中，异氟烷和七氟烷麻醉比低温麻醉更能持续维持生理参数（HR、RR、%SpO2）。
