Min Hyun Jin, Hong Seok Chan, Yang Hoon Shik, Mun Seog Kyun, Lee Sei Young
Department of Otorhinolaryngology-Head and Neck Surgery, Chung-Ang University College of Medicine, Seoul, Korea.
Yonsei Med J. 2016 Mar;57(2):469-74. doi: 10.3349/ymj.2016.57.2.469.
We tried to evaluate the difference in the expression of carbonic anhydrase (CA) III and heat shock protein (Hsp) 70 between laryngopharyngeal reflux disease (LPRD) and non-LPRD patients.
The study involved 28 patients who underwent laryngeal microsurgery due to benign laryngeal disease from March to August 2008. Reflux symptom index (RSI) and reflux finding score (RFS) were measured for each person, and they were assigned either to the LPRD group (n=10) or non-LPRD group (n=18). Tissue samples were obtained from the mucosa of posterior commissure, and immunohistochemistry (IHC) staining of CAIII and Hsp70 was performed. The IHC scores were measured and compared with clinical features including RSI and RFS.
Total 10 patients were assigned as LPRD group, and 18 patients were as control group. The mean IHC score of CAIII and Hsp70 was 1.70 ± 1.06 and 1.90 ± 0.88, respectively, in LPRD patients, whereas the mean IHC score of CAIII and Hsp70 was 0.78 ± 0.73 and 0.94 ± 0.87, respectively, in non-LPRD patients. The difference between two groups was statistically significant (p<0.05).
CAIII and Hsp70 expressions were higher in LPRD patients that in non-LPRD patients, suggesting the possibility as one of biomomarker in LPRD diagnosis.
我们试图评估喉咽反流病（LPRD）患者与非LPRD患者碳酸酐酶（CA）III和热休克蛋白（Hsp）70表达的差异。
该研究纳入了2008年3月至8月因良性喉部疾病接受喉显微手术的28例患者。测量每个人的反流症状指数（RSI）和反流发现评分（RFS），并将他们分为LPRD组（n = 10）或非LPRD组（n = 18）。从后联合黏膜获取组织样本，进行CAIII和Hsp70的免疫组织化学（IHC）染色。测量IHC评分并与包括RSI和RFS在内的临床特征进行比较。
共10例患者被分配到LPRD组，18例患者作为对照组。LPRD患者中CAIII和Hsp70的平均IHC评分分别为1.70±1.06和1.90±0.88，而非LPRD患者中CAIII和Hsp70的平均IHC评分分别为0.78±0.73和0.94±0.87。两组之间的差异具有统计学意义（p<0.05）。
LPRD患者中CAIII和Hsp70的表达高于非LPRD患者，提示其有可能作为LPRD诊断的生物标志物之一。
