Voicu Georgeta, Popa Alexandru Mihai, Badanoiu Alina Ioana, Iordache Florin
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, 1-7 Gh. Polizu Street, Bucharest RO-011061, Romania.
Department of Fetal and Adult Stem Cell Therapy, Nicolae Simionescu Institute of Cellular Biology and Pathology of Romanian Academy, 8 B.P. Hasdeu Street, Bucharest RO-050568, Romania.
Molecules. 2016 Feb 18;21(2):233. doi: 10.3390/molecules21020233.
In this study the sol-gel process was used to synthesize a precursor mixture for the preparation of silicate cement, also called mineral trioxide aggregate (MTA) cement. This mixture was thermally treated under two different conditions (1400 °C/2 h and 1450 °C/3 h) followed by rapid cooling in air. The resulted material (clinker) was ground for one hour in a laboratory planetary mill (v = 150 rot/min), in order to obtain the MTA cements. The setting time and mechanical properties, in vitro induction of apatite formation by soaking in simulated body fluid (SBF) and cytocompatibility of the MTA cements were assessed in this study. The hardening processes, nature of the reaction products and the microstructural characteristics were also investigated. The anhydrous and hydrated cements were characterized by different techniques e.g., X-ray diffraction (XRD), scanning electron microscopy (SEM), infrared spectroscopy (FT-IR) and thermal analysis (DTA-DTG-TG). The setting time of the MTA cement obtained by thermal treatment at 1400 °C/2 h (MTA1) was 55 min and 15 min for the MTA cement obtained at 1450 °C/3 h (MTA2). The compressive strength values were 18.5 MPa (MTA1) and 22.9 MPa (MTA2). Both MTA cements showed good bioactivity (assessed by an in vitro test), good cytocompatibility and stimulatory effect on the proliferation of cells.
在本研究中，采用溶胶 - 凝胶法合成了用于制备硅酸盐水泥（也称为三氧化物矿物聚集体（MTA）水泥）的前驱体混合物。该混合物在两种不同条件下（1400℃/2小时和1450℃/3小时）进行热处理，随后在空气中快速冷却。将所得材料（熟料）在实验室行星式磨机中研磨1小时（v = 150转/分钟），以获得MTA水泥。本研究评估了MTA水泥的凝结时间和力学性能、浸泡在模拟体液（SBF）中体外诱导磷灰石形成的能力以及细胞相容性。还研究了硬化过程、反应产物的性质和微观结构特征。通过不同技术对无水和水合水泥进行了表征，例如X射线衍射（XRD）、扫描电子显微镜（SEM）、红外光谱（FT - IR）和热分析（DTA - DTG - TG）。在1400℃/2小时热处理得到的MTA水泥（MTA1）的凝结时间为55分钟，在1450℃/3小时得到的MTA水泥（MTA2）的凝结时间为15分钟。抗压强度值分别为18.5MPa（MTA1）和22.9MPa（MTA2）。两种MTA水泥均表现出良好的生物活性（通过体外试验评估）、良好的细胞相容性以及对细胞增殖的刺激作用。
