Bonomo M M, Morozesk M, Duarte I D, Rocha L D, Fernandes M N, Matsumoto S T
Departamento de Ciências Biológicas, Universidade Federal do Espírito Santo (UFES), CEP 29075-910, Av. Fernando Ferrari, n 514, Vitoria, Espirito Santo, Brazil.
Departamento de Ciências Fisiológicas, Universidade Federal de São Carlos (UFSCar), CEP 13565-905, São Carlos, São Paulo, Brazil.
Environ Sci Pollut Res Int. 2016 Jun;23(11):11069-11075. doi: 10.1007/s11356-016-6201-8. Epub 2016 Feb 24.
Application of sewage sludge in agricultural lands is a growing practice in several countries due to its numerous benefits to soil and crops, where chemical and pathogen levels are determined by corresponding legislation. However, the presence of contaminants in residues must always be controlled before application due to their dangerous effects over the ecosystem and potential risks to human health. The main objective of this study was to integrate biological and chemical analysis in order to help elucidating the residue potential toxic, cytotoxic, and mutagenic effects. We evaluate samples of sewage sludge before and after the sanitizing treatment with lime in cytokinesis-block assay using CHO-k1 culture cells. The sanitizing treatment promoted a decrease in pathogen levels, which is the main purpose of this process. Even with chemical levels below the established by environmental agencies, results showed sewage sludge ability to enhance genotoxic and mutagenic effects, proving that residue should be handled with caution in order to minimize its environmental and human risk.
由于污水污泥对土壤和作物有诸多益处，在一些国家，将其应用于农田的做法日益普遍，其化学和病原体含量由相应法规确定。然而，由于残留物中的污染物对生态系统有危险影响并对人类健康存在潜在风险，在施用前必须始终对其存在情况进行控制。本研究的主要目的是整合生物和化学分析，以帮助阐明残留物潜在的毒性、细胞毒性和诱变作用。我们在使用CHO-k1培养细胞的胞质分裂阻滞试验中，评估了用石灰进行消毒处理前后的污水污泥样本。消毒处理使病原体含量降低，这是该过程的主要目的。即使化学含量低于环境机构规定的水平，结果表明污水污泥仍有增强遗传毒性和诱变作用的能力，证明应对残留物谨慎处理，以尽量减少其对环境和人类的风险。
