Department of Life, Health & Chemical Sciences, The Open University, Milton Keynes, MK7 6AA, UK.
Department of Laboratory Animal Science, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
Nanomedicine (Lond). 2016 Mar;11(6):617-25. doi: 10.2217/nnm.15.215. Epub 2016 Feb 25.
To identify the localization of glucose-coated gold nanoparticles within cells of the brain after intravascular infusion which may point to the mechanism by which they cross the blood-brain barrier.
MATERIALS & METHODS: Tissue distribution of the nanoparticles was measured by inductively-coupled-mass spectrometry and localization within the brain by histochemistry and electron microscopy.
RESULTS & CONCLUSION: Nanoparticles were identified within neurons and glial cells more than 10 μm from the nearest microvessel within 10 min of intracarotid infusion. Their distribution indicated movement across the endothelial cytosol, and direct transfer between cells of the brain. The rapid movement of this class of nanoparticle (<5 nm) into the brain demonstrates their potential to carry therapeutic biomolecules or imaging reagents.
确定血管内输注后葡萄糖包覆金纳米粒子在脑内细胞中的定位，这可能指向它们穿过血脑屏障的机制。
通过电感耦合质谱法测量纳米粒子的组织分布，并通过组织化学和电子显微镜定位其在脑内的定位。
在颈内动脉输注后 10 分钟内，在距离最近微血管 10 μm 以上的神经元和神经胶质细胞中鉴定出纳米粒子。它们的分布表明穿过内皮细胞质和脑内细胞之间的直接转移。这类纳米粒子(<5nm)迅速进入脑内，证明它们具有携带治疗性生物分子或成像试剂的潜力。
