Shah Jigna D, Desai Prerak T, Zhang Ying, Scharber Sarah K, Baller Joshua, Xing Zheng S, Cardona Carol J
Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, Minnesota, United States of America.
Microbiology and Molecular Genetics, University of California-Irvine, Irvine, California, United States of America.
PLoS One. 2016 Feb 25;11(2):e0150094. doi: 10.1371/journal.pone.0150094. eCollection 2016.
Several RNA viruses such as astrovirus, rotavirus, reovirus and parvovirus have been detected in both healthy and diseased commercial poultry flocks. The aim of this study was to characterize (a) the development of the RNA viral community in the small intestines of healthy broiler chickens from hatch through 6 weeks of age (market age) and (b) the contribution of the breeder source vs. bird age in development of the community structure. Intestinal tissue samples were harvested from breeders and their progeny, processed for viral RNA extraction and sequenced using Illumina Hiseq sequencing technology resulting in 100 bp PE reads. The results from this study indicated that the breeder source influenced the RNA viral community only at hatch but later environment i.e. bird age had the more significant effect. The most abundant RNA viral family detected at 2, 4 and 6 weeks of age was Astroviridae, which decreased in abundance with age while the abundance of Picornaviridae increased with age.
在健康和患病的商业家禽群中均检测到了多种RNA病毒，如星状病毒、轮状病毒、呼肠孤病毒和细小病毒。本研究的目的是：（a）描述从孵化到6周龄（上市年龄）的健康肉鸡小肠中RNA病毒群落的发展情况；（b）种鸡来源与鸡龄对群落结构发展的贡献。从种鸡及其后代采集肠道组织样本，进行处理以提取病毒RNA，并使用Illumina Hiseq测序技术进行测序，产生100 bp的双端 reads。本研究结果表明，种鸡来源仅在孵化时影响RNA病毒群落，但后期环境（即鸡龄）的影响更为显著。在2周龄、4周龄和6周龄时检测到的最丰富的RNA病毒科是星状病毒科，其丰度随年龄增长而降低，而小RNA病毒科的丰度随年龄增长而增加。
