长链非编码RNA ucoo2kmd.1通过竞争结合miR-211-3p调控结直肠癌中CD44依赖的细胞生长
Long Non-Coding RNA ucoo2kmd.1 Regulates CD44-Dependent Cell Growth by Competing for miR-211-3p in Colorectal Cancer.
作者信息
Wu Xiaoli, He Xixi, Li Shi, Xu Xiaoqun, Chen Xiangjian, Zhu Hua
机构信息
Department of gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, P.R. China.
出版信息
PLoS One. 2016 Mar 14;11(3):e0151287. doi: 10.1371/journal.pone.0151287. eCollection 2016.
In addition to protein-coding genes, the human genome makes a large amount of noncoding RNAs. Long non-coding RNAs (lncRNAs) have been described as the largest subclass of the non-coding transcriptome in human noncoding RNAs. In recent years, lncRNAs have been considered to be the key regulators of tumor behavior. In this study, based on previous research, we investigated the expression and biological role of a newly identified cancer-related lncRNA, lncRNA-uc002kmd.1. We analyzed the relationship between lncRNA-uc002kmd.1 and colorectal cancer (CRC) in a total 45 CRC and paired adjacent, non-tumor tissue samples. We found that lncRNA-uc002kmd.1 expression was usually highly expressed in carcinoma compared with the tissue adjacent to the carcinoma. Through a series of experiments, the results showed that lncRNA-uc002kmd.1 regulates CD44 as a molecular decoy for miR211-3p. Our data indicated that the overexpression of lncRNA-uc002kmd.1 enhanced cell proliferation in CRC.
除了蛋白质编码基因外，人类基因组还产生大量非编码RNA。长链非编码RNA（lncRNA）被认为是人类非编码RNA中转录组的最大亚类。近年来，lncRNA被认为是肿瘤行为的关键调节因子。在本研究中，基于之前的研究，我们调查了一种新发现的癌症相关lncRNA，即lncRNA-uc002kmd.1的表达及其生物学作用。我们分析了45例结直肠癌（CRC）及配对的相邻非肿瘤组织样本中lncRNA-uc002kmd.1与结直肠癌的关系。我们发现，与癌旁组织相比，lncRNA-uc002kmd.1在癌组织中通常高表达。通过一系列实验，结果表明lncRNA-uc002kmd.1作为miR211-3p的分子诱饵调节CD44。我们的数据表明，lncRNA-uc002kmd.1的过表达增强了结直肠癌中的细胞增殖。
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