Subedi Nirmal Kumar, Rahman S M Abdur, Akbar Mohammad Ahsanul
Faculty of Pharmacy, Little Buddha College of Health Science, Purbanchal University, Kathmandu 44600, Nepal.
Department of Clinical Pharmacy & Pharmacology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Evid Based Complement Alternat Med. 2016;2016:3820704. doi: 10.1155/2016/3820704. Epub 2016 Feb 11.
The study aims to evaluate analgesic and antipyretic activities of the methanol extract and its different fractions from root of Schoenoplectus grossus using acetic acid induced writhing and radiant heat tail flick method of pain models in mice and yeast induced pyrexia in rats at the doses of 400 and 200 mg/kg. In acetic acid writhing test, the methanol extract, petroleum ether, and carbon tetrachloride fractions produced significant (P < 0.001 and P < 0.05) inhibition of writhing responses in dose dependent manner. The methanol extract at 400 and 200 mg/kg being more protective with 54% and 45.45% of inhibition compared to diclofenac sodium of 56% followed by petroleum ether fractions of 49.69% and 39.39% at the same doses. The extracts did not produce any significant antinociceptive activity in tail flick test except standard morphine. When studied on yeast induced pyrexia, methanol and petroleum ether fractions significantly lowered the rectal temperature time dependently in a manner similar to standard drug paracetamol and distinctly more significant (P < 0.001) after second hour. These findings suggest that the root extracts of S. grossus possess significant peripherally acting analgesic potential and antipyretic property. The phytochemical screening showed the presence of flavonoids, alkaloids, and tannins.
本研究旨在采用乙酸诱导小鼠扭体和辐射热甩尾疼痛模型以及酵母诱导大鼠发热模型，在400和200mg/kg剂量下，评估粗状荸荠根甲醇提取物及其不同馏分的镇痛和解热活性。在乙酸扭体试验中，甲醇提取物、石油醚和四氯化碳馏分以剂量依赖性方式对扭体反应产生显著（P<0.001和P<0.05）抑制作用。400和200mg/kg的甲醇提取物具有更强的保护作用，抑制率分别为54%和45.45%，而双氯芬酸钠为56%，相同剂量下石油醚馏分为49.69%和39.39%。除标准吗啡外，提取物在甩尾试验中未产生任何显著的抗伤害感受活性。在酵母诱导发热试验中，甲醇和石油醚馏分能使直肠温度随时间显著降低，其方式与标准药物对乙酰氨基酚相似，且在第二小时后作用更显著（P<0.001）。这些结果表明，粗状荸荠根提取物具有显著的外周作用镇痛潜力和解热特性。植物化学筛选显示存在黄酮类、生物碱和单宁。
