Pullman James M, Nylk Jonathan, Campbell Elaine C, Gunn-Moore Frank J, Prystowsky Michael B, Dholakia Kishan
Department of Pathology, Montefiore Medical Center and Albert Einstein College of Medicine, Bronx, NY, USA.
School of Biology, University of St Andrews, St Andrews, KY16 9FT, UK; SUPA, School of Physics and Astronomy, University of St Andrews, St Andrews, KY16 9SS, UK.
Biomed Opt Express. 2016 Jan 6;7(2):302-11. doi: 10.1364/BOE.7.000302. eCollection 2016 Feb 1.
A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease.
对肾足细胞亚结构进行详细的微观分析对于理解和诊断肾病至关重要。目前，只有耗时的电子显微镜（EM）才能解析这种亚结构。我们使用结构照明显微镜（SIM）检查用足突蛋白免疫荧光标记染色的肾活检冰冻切片，足突蛋白是一种定位于足细胞足突周边的蛋白质，并将正常和肾病活检中的结果与EM进行比较。正常肾小球的SIM图像显示足突蛋白的曲线模式紧密覆盖毛细血管壁，类似于EM所见的足细胞足突。所有肾病活检的足突蛋白染色与正常情况有显著差异，与EM所见的足突消失相对应且能更好地显示。这些发现支持了SIM在肾病诊断中的首次潜在应用。
