Aeschbacher M, Piffaretti J C
Istituto Cantonale Batteriologico, Lugano, Switzerland.
Infect Immun. 1989 May;57(5):1432-7. doi: 10.1128/iai.57.5.1432-1437.1989.
A total of 125 strains of Campylobacter jejuni and Campylobacter coli from human and animal hosts were analyzed by multilocus enzyme electrophoresis. Ten genetic loci were characterized by the electrophoretic mobilities of their products, and all were found to be polymorphic. The nine loci considered for genetic analysis had an average allele number of 6.9 and a mean genetic diversity of 0.634. The strains were grouped into 64 electrophoretic types (ETs) forming two principal clusters; the first cluster consisted of 50 ETs represented by 104 isolates of C.jejuni, and the second included 14 ETs represented by 21 isolates of C. coli. The genetic distance between the two species was 0.939. The data support the conclusions that (i) campylobacteriosis caused by C. jejuni and C. coli is a zoonosis, (ii) human and animal strains do not constitute subpopulations, and (iii) every animal strain may be considered a potential human pathogen. The frequency of intraspecies gene transfer seems to be moderately high whereas interspecies gene transfer is very rare at best, which suggests the existence of a biological barrier between C. jejuni and C. coli.
采用多位点酶电泳法对来自人类和动物宿主的125株空肠弯曲菌和结肠弯曲菌进行了分析。通过其产物的电泳迁移率对10个基因位点进行了表征，发现所有位点均具有多态性。用于遗传分析的9个位点的平均等位基因数为6.9，平均遗传多样性为0.634。这些菌株被分为64种电泳类型（ETs），形成两个主要聚类；第一个聚类由50种ETs组成，由104株空肠弯曲菌分离株代表，第二个聚类包括14种ETs，由21株结肠弯曲菌分离株代表。两个物种之间的遗传距离为0.939。这些数据支持以下结论：（i）由空肠弯曲菌和结肠弯曲菌引起的弯曲菌病是人畜共患病，（ii）人类和动物菌株不构成亚群，（iii）每一株动物菌株都可被视为潜在的人类病原体。种内基因转移的频率似乎适中偏高，而种间基因转移充其量非常罕见，这表明空肠弯曲菌和结肠弯曲菌之间存在生物屏障。
