Tolstik Elen, Osminkina Liubov Andreevna, Matthäus Christian, Burkhardt Markus, Tsurikov Konstantine Eduardovich, Natashina Uliana Alexandrovna, Timoshenko Victor Yurievich, Heintzmann Rainer, Popp Juergen, Sivakov Vladimir
Leibniz Institute of Photonic Technology, Jena, Germany; Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-Schiller-University, Jena Germany.
Lomonosov Moscow State University, Physics Department, Moscow, Russia; National Research Nuclear University MEPhI, Moscow, Russia.
Nanomedicine. 2016 Oct;12(7):1931-1940. doi: 10.1016/j.nano.2016.04.004. Epub 2016 Apr 13.
In-vitro Raman micro-spectroscopy was used for diagnostics of the processes of uptake and biodegradation of porous silicon nanoparticles (SiNPs) in breast cancer cells (MCF-7 cell line). Two types of nanoparticles, with and without photoluminescence in the visible spectral range, were investigated. The spatial distribution of photoluminescent SiNPs within the cells obtained by Raman imaging was verified by high-resolution structured-illumination optical microscopy. Nearly complete biodegradation of SiNPs inside the living cells was observed after 13days of the incubation. The results reveal new prospects of multi-modal visualization of SiNPs inside cancer cells for theranostic applications.
体外拉曼显微光谱用于诊断乳腺癌细胞（MCF-7细胞系）对多孔硅纳米颗粒（SiNPs）的摄取和生物降解过程。研究了两种类型的纳米颗粒，一种在可见光谱范围内有光致发光，另一种没有。通过拉曼成像获得的细胞内光致发光SiNPs的空间分布通过高分辨率结构照明显微镜进行了验证。孵育13天后，观察到活细胞内的SiNPs几乎完全生物降解。这些结果揭示了癌细胞内SiNPs用于治疗诊断应用的多模态可视化的新前景。
