Department of Industrial Engineering, University of Padova, Padova, Italy.
Venetian Institute of Molecular Medicine (VIMM), Padova, Italy.
Nat Methods. 2016 May;13(5):446-52. doi: 10.1038/nmeth.3832. Epub 2016 Apr 18.
We report that the efficiency of reprogramming human somatic cells to induced pluripotent stem cells (hiPSCs) can be dramatically improved in a microfluidic environment. Microliter-volume confinement resulted in a 50-fold increase in efficiency over traditional reprogramming by delivery of synthetic mRNAs encoding transcription factors. In these small volumes, extracellular components of the TGF-β and other signaling pathways exhibited temporal regulation that appears critical to acquisition of pluripotency. The high quality and purity of the resulting hiPSCs (μ-hiPSCs) allowed direct differentiation into functional hepatocyte- and cardiomyocyte-like cells in the same platform without additional expansion.
我们报告称，在微流控环境中，可极大地提高将人类体细胞重编程为诱导多能干细胞（hiPSC）的效率。与传统的通过递送编码转录因子的合成 mRNA 进行重编程相比，微升体积限制使效率提高了 50 倍。在这些小体积中，TGF-β 和其他信号通路的细胞外成分表现出时间调节，这似乎对获得多能性至关重要。所得 hiPSC（μ-hiPSC）的高质量和高纯度允许在同一平台上直接分化为功能性肝细胞和心肌细胞样细胞，而无需额外的扩增。
