Charan S S, Pawar K D, Gavhale S D, Tikhe C V, Charan N S, Angel B, Joshi V, Patole M S, Shouche Y S
Molecular Biology Unit, National Centre for Cell Science, Pune, Maharashtra, India.
Desert Medicine Research Centre, Jodhpur, Rajasthan, India.
Med Vet Entomol. 2016 Sep;30(3):264-77. doi: 10.1111/mve.12173. Epub 2016 Apr 20.
Dengue viruses are transmitted to humans through the bites of infected female aedine mosquitoes. Differences in the composition and structure of bacterial communities in the midguts of mosquitoes may affect the vector's ability to transmit the disease. To investigate and analyse the role of midgut bacterial communities in viral transmission, midgut bacteria from three species, namely Stegomyia aegypti (= Aedes aegypti), Fredwardsius vittatus (= Aedes vittatus) and Stegomyia albopicta (= Aedes albopictus) (all: Diptera: Culicidae), from dengue-endemic and non-endemic areas of Rajasthan, India were compared. Construction and analyses of six 16S rRNA gene libraries indicated that Serratia spp.-related phylotypes dominated all clone libraries of the three mosquito species from areas in which dengue is not endemic. In dengue-endemic areas, phylotypes related to Aeromonas, Enhydrobacter spp. and uncultivated bacterium dominated the clone libraries of S. aegypti, F. vittatus and S. albopicta, respectively. Diversity indices analysis and real-time TaqMan polymerase chain reaction assays showed bacterial diversity and abundance in the midguts of S. aegypti to be higher than in the other two species. Significant differences observed among midgut bacterial communities of the three mosquito species from areas in which dengue is and is not endemic, respectively, may be related to the vectorial capacity of mosquitoes to carry dengue viruses and, hence, to the prevalence of disease in some areas.
登革病毒通过受感染的雌性伊蚊叮咬传播给人类。蚊子中肠细菌群落的组成和结构差异可能会影响病媒传播疾病的能力。为了调查和分析中肠细菌群落在病毒传播中的作用，对来自印度拉贾斯坦邦登革热流行区和非流行区的埃及伊蚊（=白纹伊蚊）、条带伊蚊（=致倦库蚊）和白纹伊蚊（=白纹伊蚊）（均为双翅目：蚊科）这三种蚊子的中肠细菌进行了比较。六个16S rRNA基因文库的构建和分析表明，在登革热非流行区的这三种蚊子的所有克隆文库中，与沙雷氏菌属相关的系统发育型占主导地位。在登革热流行区，与气单胞菌属、嗜水气单胞菌属和未培养细菌相关的系统发育型分别在埃及伊蚊、条带伊蚊和白纹伊蚊的克隆文库中占主导地位。多样性指数分析和实时TaqMan聚合酶链反应分析表明，埃及伊蚊中肠的细菌多样性和丰度高于其他两个物种。分别在登革热流行区和非流行区的这三种蚊子的中肠细菌群落之间观察到的显著差异，可能与蚊子携带登革病毒的病媒能力有关，因此也与某些地区的疾病流行率有关。
