Coatsworth Heather, Caicedo Paola A, Van Rossum Thea, Ocampo Clara B, Lowenberger Carl
C2D2 Research Group, Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.
Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Cali, Colombia.
J Insect Sci. 2018 Nov 1;18(6):12. doi: 10.1093/jisesa/iey118.
The composition, abundance, and diversity of midgut bacteria in mosquitoes can influence pathogen transmission. We used 16S rRNA microbiome profiling to survey midgut microbial diversity in pooled samples of laboratory colonized dengue-refractory, Cali-MIB, and dengue-susceptible, Cali-S Aedes aegypti (Linnaeus). The 16S rRNA sequences from the sugar-fed midguts of adult females clustered to 63 amplicon sequence variants (ASVs), primarily from Proteobacteria, Firmicutes, Flavobacteria, and Actinobacteria. An average of five ASVs dominated the midguts, and most ASVs were present in both Cali-MIB and Cali-S midguts. No differences in abundance were noted at any phylogenetic level (Phylum, Class, Order, Family, Genus) by analysis of composition of microbiome (w = 0). No community diversity metrics were significantly different between refractory and susceptible mosquitoes. These data suggest that phenotypic differences in the susceptibility to dengue virus between Cali-MIB and Cali-S are not likely due to major differences in midgut bacterial communities.
蚊子中肠细菌的组成、丰度和多样性会影响病原体传播。我们使用16S rRNA微生物组分析方法，对实验室饲养的登革热抗性品系Cali-MIB和登革热易感品系Cali-S的埃及伊蚊（林奈）混合样本的中肠微生物多样性进行了调查。成年雌性埃及伊蚊取食糖水后中肠的16S rRNA序列聚类为63个扩增子序列变体（ASV），主要来自变形菌门、厚壁菌门、黄杆菌门和放线菌门。平均有5个ASV在中肠中占主导地位，并且大多数ASV在Cali-MIB和Cali-S的中肠中都存在。通过微生物组组成分析（w = 0），在任何系统发育水平（门、纲、目、科、属）均未发现丰度差异。抗性蚊子和易感蚊子之间的群落多样性指标没有显著差异。这些数据表明，Cali-MIB和Cali-S对登革病毒易感性的表型差异不太可能是由于中肠细菌群落的主要差异所致。
