Fæste Christiane Kruse, Jonscher Karen R, Dooper Maaike M W B, Egge-Jacobsen Wolfgang, Moen Anders, Daschner Alvaro, Egaas Eliann, Christians Uwe
Norwegian Veterinary Institute, Oslo, Norway.
University of Colorado Denver, Aurora, CO, USA.
EuPA Open Proteom. 2014 Sep;4:140-155. doi: 10.1016/j.euprot.2014.06.006.
The parasitic nematode occurs in fish stocks in temperate seas. contamination of fish products is unsavoury and a health concern considering human infection with live larvae (anisakiasis) and allergic reactions to anisakid proteins in seafood. Protein extracts of produce complex band patterns in gel electrophoresis and IgE-immunostaining. In the present study potential allergens have been characterised using sera from -sensitised patients and proteome data obtained by mass spectrometry. proteins were homologous to allergens in other nematodes, insects, and shellfish indicating cross-reactivity. Characteristic marker peptides for relevant proteins were described.
这种寄生线虫存在于温带海域的鱼类种群中。考虑到人类感染活幼虫（异尖线虫病）以及对海鲜中异尖线虫蛋白的过敏反应，鱼产品受到污染既令人不快又关乎健康。[该线虫]的蛋白质提取物在凝胶电泳和免疫球蛋白E免疫染色中产生复杂的条带模式。在本研究中，利用对[该线虫]致敏患者的血清以及通过质谱法获得的蛋白质组数据对潜在过敏原进行了表征。[该线虫的]蛋白质与其他线虫、昆虫和贝类中的过敏原同源，表明存在交叉反应。描述了相关[该线虫]蛋白质的特征性标记肽。
