Amrani Amira, van Helden Jacques, Bergon Aurélie, Aouane Aicha, Ben Hania Wajdi, Tamburini Christian, Loriod Béatrice, Imbert Jean, Ollivier Bernard, Pradel Nathalie, Dolla Alain
Aix-Marseille Université, Université du Sud Toulon-Var, IRD, CNRS/INSU, MIO, UM110, Marseille, Cedex 09, 13288, France.
Aix-Marseille Université, CNRS, LCB-UMR7283, Marseille, France.
Environ Microbiol Rep. 2016 Aug;8(4):520-6. doi: 10.1111/1758-2229.12427.
Desulfovibrio piezophilus strain C1TLV30(T) is a mesophilic piezophilic sulfate-reducer isolated from Wood Falls at 1700 m depth in the Mediterranean Sea. In this study, we analysed the effect of the hydrostatic pressure on this deep-sea living bacterium at the physiologic and transcriptomic levels. Our results showed that lactate oxidation and energy metabolism were affected by the hydrostatic pressure. Especially, acetyl-CoA oxidation pathway and energy conservation through hydrogen and formate recycling would be more important when the hydrostatic pressure is above (26 MPa) than below (0.1 MPa) the optimal one (10 MPa). This work underlines also the role of the amino acid glutamate as a piezolyte for the Desulfovibrio genus. The transcriptomic analysis revealed 146 differentially expressed genes emphasizing energy production and conversion, amino acid transport and metabolism and cell motility and signal transduction mechanisms as hydrostatic pressure responding processes. This dataset allowed us to identify a sequence motif upstream of a subset of differentially expressed genes as putative pressure-dependent regulatory element.
嗜压脱硫弧菌菌株C1TLV30(T)是一种嗜温嗜压硫酸盐还原菌，从地中海1700米深处的伍德瀑布分离得到。在本研究中，我们在生理和转录组水平分析了静水压力对这种深海生存细菌的影响。我们的结果表明，乳酸氧化和能量代谢受静水压力影响。特别是，当静水压力高于（26 MPa）而非低于（0.1 MPa）最佳压力（10 MPa）时，乙酰辅酶A氧化途径以及通过氢气和甲酸盐循环进行的能量守恒更为重要。这项工作还强调了氨基酸谷氨酸作为脱硫弧菌属的一种耐压溶质的作用。转录组分析揭示了146个差异表达基因，这些基因强调了作为静水压力响应过程的能量产生和转换、氨基酸运输和代谢以及细胞运动和信号转导机制。该数据集使我们能够鉴定差异表达基因子集上游的一个序列基序，作为假定的压力依赖性调控元件。
