Andrewartha Sarah J, Munns Suzanne L, Edwards Ashley
CSIRO Agriculture, Integrated and Sustainable Aquaculture Production, Castray Esplanade, Hobart, TAS 7000, Australia; Institute of Marine and Antarctic Science, University of Tasmania, Castray Esplanade, Hobart, TAS 7000, Australia.
Biomedical Sciences, College of Public Health Medical and Veterinary Sciences, James Cook University, Townsville, QLD 4812, Australia.
Conserv Physiol. 2016 Mar 12;4(1):cow006. doi: 10.1093/conphys/cow006. eCollection 2016.
Haemoglobin concentration ([Hb]) is measured for a wide variety of animal studies. The use of point-of-care devices, such as the HemoCue, is becoming increasingly common because of their portability, relative ease of use and low cost. In this study, we aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be determined accurately using the HemoCue and whether the HemoCue overestimates the [Hb] of reptile blood in a similar manner to fish blood. Additionally, we aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201(+) systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon, and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species and, as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations.
血红蛋白浓度（[Hb]）在各种动物研究中都有测量。由于即时检测设备（如HemoCue）具有便携性、相对易于使用和低成本等特点，其使用越来越普遍。在本研究中，我们旨在确定是否可以使用HemoCue准确测定蓝舌石龙子（Tiliqua nigrolutea）血液的[Hb]，以及HemoCue是否会像对待鱼血那样高估爬行动物血液的[Hb]。此外，我们旨在测试倍性是否会影响使用二倍体和三倍体大西洋鲑（Salmo salar）血液通过HemoCue测定的[Hb]。HemoCue Hb 201(+)系统地高估了蓝舌石龙子和大西洋鲑的[Hb]，并且为二倍体或三倍体鲑鱼确定的校准方程之间没有差异。这种高估在两个物种中都是系统性的，因此，可以使用适当的校准方程对通过HemoCue测定的[Hb]进行校正。
