Department of Biological Sciences, University of Bergen, Bergen, Norway.
Faculty of Biosciences and Aquaculture, Nord University, 8049, Bodø, Norway.
Fish Physiol Biochem. 2019 Oct;45(5):1551-1562. doi: 10.1007/s10695-019-00638-1. Epub 2019 Oct 3.
Portable clinical analysers are gradually being involved in on-site assessment of haematic parameters in fish. The purpose of this study was to evaluate the i-STAT portable clinical analyser (i-STAT PCA) for accuracy and reliability of measuring blood pH, partial pressure of oxygen (pO), haematocrit, haemoglobin, sodium, potassium and calcium in Atlantic cod (Gadus morhua). Haematological parameters detected with the i-STAT PCA were compared with conventional laboratory techniques (CLTs). Two types of disposable cartridges were used (CHEM8+ and CG4+) with the i-STAT PCA, and experiments were performed at two different temperature regimes (5 °C and 15 °C) and four different carbon dioxide (CO) levels (0%, 0.1%, 0.5% and 1%). All blood parameters measured with the i-STAT PCA showed heterogeneous inaccuracy under the tested conditions, but the highest discrepancies were registered in blood pO. The i-STAT PCA systematically overestimated the pO measurements. Our research suggests that i-STAT PCA is not an appropriate tool for pO measurements especially in coldwater fish species. The i-STAT PCA consistently underestimated the pH and haematocrit values especially at a lower temperature, although those parameters indicate significant high correlation at 15 °C. Furthermore, the analysed ions showed overestimation of sodium and underestimation of potassium and calcium.
便携式临床分析仪逐渐被应用于鱼类血液参数的现场评估。本研究旨在评估 i-STAT 便携式临床分析仪（i-STAT PCA）在测量大西洋鳕鱼（Gadus morhua）血液 pH 值、部分氧气压力（pO）、红细胞压积、血红蛋白、钠、钾和钙方面的准确性和可靠性。使用 i-STAT PCA 检测的血液参数与传统实验室技术（CLTs）进行了比较。使用 i-STAT PCA 两种类型的即用型试剂盒（CHEM8+ 和 CG4+），并在两个不同温度（5°C 和 15°C）和四个不同二氧化碳（CO）水平（0%、0.1%、0.5%和 1%）下进行了实验。在测试条件下，i-STAT PCA 测量的所有血液参数均表现出不均匀的不准确性，但在血液 pO 方面的差异最大。i-STAT PCA 系统地高估了 pO 的测量值。我们的研究表明，i-STAT PCA 不是测量 pO 的合适工具，特别是在冷水鱼类中。i-STAT PCA 一直低估 pH 值和红细胞压积值，特别是在较低温度下，尽管这些参数在 15°C 时显示出显著的高相关性。此外，分析的离子显示出对钠的高估和对钾和钙的低估。
