Barel A O, Clarys P
Laboratory of General and Biological Chemistry, Faculty of Physical Education and Physiotherapy, Free University of Brussels, Brussels, Belgium.
Skin Res Technol. 1997 May;3(2):107-13. doi: 10.1111/j.1600-0846.1997.tb00171.x.
BACKGROUND/AIMS: A major problem with electrical measurements of the capacitance of the skin using the well kwown capacitance method (Corneometer) resides in the fact that the results of this instrument are expressed as arbitrary capacitance hydration units that are not directly related to real electrical units or to the water content of the horny layer. The purpose of this study was to establish a calibration of the capacitance method using a simple in vitro simulation system of the horny layer.
The calibration of capacitance and conductance methods was carried out using cellulose filter papers saturated with water, aqueous solutions and liquids of different dielectric constant (sorption and desorption experiments).
Using this in vitro method, correlations were established between the capacitance values and the quantity of water adsorbed on the filter and with the dielectric constant of the liquid saturating the filter paper. High correlations were observed during sorption and desorption experiments between the capacitance and conductance measurements.
In vitro calibration of the capacitance method is now possible with this simulation model of the horny layer. Furthermore, determination of the range of sensitivity, accuracy, repeatability and depth of detection of the measuring probe of the capacitance and conductance method were carried out.
背景/目的：使用广为人知的电容法（角质层水合测量仪）对皮肤电容进行电学测量的一个主要问题在于，该仪器的结果是以任意的电容水合单位表示的，这些单位与实际电学单位或角质层的含水量没有直接关系。本研究的目的是使用角质层的简单体外模拟系统建立电容法的校准。
使用用水、水溶液和不同介电常数的液体饱和的纤维素滤纸进行电容和电导法的校准（吸附和解吸实验）。
使用这种体外方法，在电容值与滤纸上吸附的水量以及饱和滤纸的液体的介电常数之间建立了相关性。在吸附和解吸实验期间，电容和电导测量之间观察到高度相关性。
现在使用这种角质层模拟模型可以对电容法进行体外校准。此外，还对电容和电导法测量探头的灵敏度范围、准确性、可重复性和检测深度进行了测定。
