Schmidt Kristina Maria, Mühlberger Elke
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Novel and Emerging Infectious Diseases, Greifswald-Insel Riems 17493, Germany.
Department of Microbiology, School of Medicine, Boston University, 620 Albany Street, Boston, MA 02118, USA.
Viruses. 2016 Jun 22;8(6):178. doi: 10.3390/v8060178.
The highly pathogenic Marburg virus (MARV) is a member of the Filoviridae family and belongs to the group of nonsegmented negative-strand RNA viruses. Reverse genetics systems established for MARV have been used to study various aspects of the viral replication cycle, analyze host responses, image viral infection, and screen for antivirals. This article provides an overview of the currently established MARV reverse genetic systems based on minigenomes, infectious virus-like particles and full-length clones, and the research that has been conducted using these systems.
高致病性马尔堡病毒（MARV）是丝状病毒科的成员，属于非节段性负链RNA病毒组。为马尔堡病毒建立的反向遗传学系统已被用于研究病毒复制周期的各个方面、分析宿主反应、对病毒感染进行成像以及筛选抗病毒药物。本文概述了目前基于微型基因组、感染性病毒样颗粒和全长克隆建立的马尔堡病毒反向遗传学系统，以及使用这些系统进行的研究。
