Toita Riki, Tsuru Kanji, Ishikawa Kunio
Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka, 563-8577, Japan.
J Mater Sci Mater Med. 2016 Aug;27(8):127. doi: 10.1007/s10856-016-5741-2. Epub 2016 Jun 25.
Bone-forming cells and Mϕ play key roles in bone tissue repair. In this study, we prepared a superhydrophilic titanium implant functionalized by ozone gas to modulate osteoconductivity and inhibit inflammatory response towards titanium implants. After 24 h of ozone gas treatment, the water contact angle of the titanium surface became zero. XPS analysis revealed that hydroxyl groups were greatly increased, but carbon contaminants were largely decreased 24 h after ozone gas functionalization. Also, ozone gas functionalization did not alter titanium surface topography. Superhydrophilic titanium (O3-Ti) largely increased the aspect ratio, size and perimeter of cells when compared with untreated titanium (unTi). In addition, O3-Ti facilitated rat bone marrow derived MSCs differentiation and mineralization evidenced by greater ALP activity and bone-like nodule formation. Interestingly, O3-Ti did not affect RAW264.7 Mϕ proliferation. However, naive RAW264.7 Mϕ cultured on unTi produced a two-fold larger amount of TNFα than that on O3-Ti. Furthermore, O3-Ti greatly mitigated proinflammatory cytokine production, including TNFα and IL-6 from LSP-stimulated RAW264.7 Mϕ. These results demonstrated that a superhydrophilic titanium prepared by simple ozone gas functionalization successfully increased MSCs proliferation and differentiation, and mitigated proinflammatory cytokine production from both naive and LPS-stimulated Mϕ. This superhydrophilic surface would be useful as an endosseous implantable biomaterials and as a biomaterial for implantation into other tissues.
成骨细胞和巨噬细胞在骨组织修复中发挥着关键作用。在本研究中，我们制备了一种经臭氧气体功能化的超亲水性钛植入物，以调节骨传导性并抑制对钛植入物的炎症反应。经过24小时的臭氧气体处理后，钛表面的水接触角变为零。X射线光电子能谱分析表明，羟基大量增加，但在臭氧气体功能化24小时后碳污染物大幅减少。此外，臭氧气体功能化并未改变钛表面形貌。与未处理的钛（unTi）相比，超亲水性钛（O3-Ti）大大增加了细胞的长宽比、大小和周长。此外，O3-Ti促进了大鼠骨髓来源的间充质干细胞的分化和矿化，碱性磷酸酶活性增强和骨样结节形成证明了这一点。有趣的是，O3-Ti不影响RAW264.7巨噬细胞的增殖。然而，在unTi上培养的原始RAW264.7巨噬细胞产生的TNFα量是在O3-Ti上培养的两倍。此外，O3-Ti大大减轻了促炎细胞因子的产生，包括来自脂多糖刺激的RAW264.7巨噬细胞的TNFα和IL-6。这些结果表明，通过简单的臭氧气体功能化制备的超亲水性钛成功地增加了间充质干细胞的增殖和分化，并减轻了原始和脂多糖刺激的巨噬细胞产生的促炎细胞因子。这种超亲水表面将作为骨内可植入生物材料以及植入其他组织的生物材料有用。
