Halifu Y, Liang J Q, Zeng X W, Ding Y, Zhang X Y, Jin T B, Yakeya B, Abudu D, Zhou Y M, Liu X M, Hu F X, Chai L, Kang X J
Department of Dermatology, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumchi, China.
Department of Plastic Surgery, The People's Hospital of Xinjiang Uyghur Autonomous Region, Urumchi, China.
Genet Mol Res. 2016 Jun 20;15(2):gmr8187. doi: 10.4238/gmr.15028187.
The Wnt signaling pathway plays a key role in insurgence and progression of many different forms of cancer. Some crucial components of the Wnt pathway have been proposed to be novel targets for cancer therapy. To date, the Wnt signaling pathway has not been studied in cutaneous squamous cell carcinoma (CSCC). This study was designed to investigate the expression of Wnt1 and SFRP1 from the Wnt pathway in CSCC. Tissue samples were obtained from 35 patients with CSCC and 30 controls admitted to the Xinjiang Uygur Autonomous Region People's Hospital at Urumchi City, China. Gene and protein expressions of Wnt1 and SFRP1 were quantified by immunohistochemistry and western blotting. Wnt1 expression was significantly higher (P < 0.05) in CSCC samples than in normal skin cells of the control subjects; in contrast, SFRP1 expression was significantly lower in CSCC tissues than that in tissues of control subjects (P < 0.05). Moreover, Wnt1 expression (P < 0.05) was found to be correlated with histopathological differentiation in CSCC, and negatively correlated with SFRP1 expression in CSCC (rs = -0.473, P = 0.015). Therefore, we concluded that Wnt1 and SFRP1 play important roles in the development of CSCC and could be potent markers for diagnosis, prevention, and therapy of CSCC.
Wnt信号通路在多种不同类型癌症的发生和发展过程中起着关键作用。Wnt通路的一些关键成分已被提议作为癌症治疗的新靶点。迄今为止，尚未对皮肤鳞状细胞癌（CSCC）中的Wnt信号通路进行研究。本研究旨在调查CSCC中Wnt通路的Wnt1和SFRP1的表达情况。组织样本取自中国乌鲁木齐市新疆维吾尔自治区人民医院收治的35例CSCC患者和30例对照者。通过免疫组织化学和蛋白质印迹法对Wnt1和SFRP1的基因和蛋白表达进行定量分析。CSCC样本中Wnt1的表达显著高于对照者的正常皮肤细胞（P < 0.05）；相反，CSCC组织中SFRP1的表达显著低于对照者组织（P < 0.05）。此外，发现CSCC中Wnt1的表达（P < 0.05）与组织病理学分化相关，且与CSCC中SFRP1的表达呈负相关（rs = -0.473，P = 0.015）。因此，我们得出结论，Wnt1和SFRP1在CSCC的发生发展中起重要作用，可能是CSCC诊断、预防和治疗的有效标志物。
