Aghaie Afsaneh, Aaskov John, Chinikar Sadegh, Niedrig Matthias, Banazadeh Soudabeh, Mohammadpour Hashem Khorsand
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, P.O. Box 14665-1157, Tehran, Iran.
Queensland University of Technology, Brisbane, Australia.
Indian J Hematol Blood Transfus. 2016 Sep;32(3):343-6. doi: 10.1007/s12288-015-0567-5. Epub 2015 Jul 30.
West Nile virus (WNV) can be transmitted by blood transfusions and organ transplants. This study was a retrospective study which was performed in Blood Transfusion Center to evaluate the WNV infection in blood donors in Iran. A total of 540 blood samples were taken from volunteer healthy donors who referred for blood donation to Chabahar Blood Center. The presence of WNV was studied by detecting immunoglobulin G (IgG) WNV by enzyme linked immune sorbent assay (ELISA). Demonstration of elevated WNV IgG confirmed by immunoflouorescence assay (IFA) Euroimmun kit. Out of the 540 samples 17.96 % (97 cases) were seropositive by ELISA and 1.48 % (8 cases) was seropositive by IFA. This means that 8.24 % of ELISA seropositive samples were confirmed by IFA. Special attention should be paid to criteria of donor selection, albeit positive results may be due to a previous infection in these donors.
西尼罗河病毒（WNV）可通过输血和器官移植传播。本研究是在输血中心进行的一项回顾性研究，旨在评估伊朗献血者中的WNV感染情况。总共从前往恰巴哈尔血液中心献血的健康志愿者中采集了540份血样。通过酶联免疫吸附测定（ELISA）检测免疫球蛋白G（IgG）WNV来研究WNV的存在情况。采用欧洲免疫（Euroimmun）试剂盒通过免疫荧光测定（IFA）确认WNV IgG升高。在540份样本中，ELISA检测显示17.96%（97例）血清呈阳性，IFA检测显示1.48%（8例）血清呈阳性。这意味着8.24%的ELISA血清阳性样本通过IFA得到了确认。尽管阳性结果可能是由于这些献血者先前感染所致，但仍应特别关注献血者选择标准。
