Chen Hong, Fan Yujuan, Xu Wensheng, Chen Junying, Xu Chaohuan, Wei Xiaoning, Fang Di, Feng Yi
Department of Gynaecology, The First Affiliated Hospital of GuangXi Medical University , Nanning, Guangxi Province, The People's Republic of China .
Cancer Biother Radiopharm. 2016 Aug;31(6):225-31. doi: 10.1089/cbr.2016.1998.
MicroRNAs are small RNA that are tightly interrelated with the initiation, development, and metastasis of cancers. Studies have shown that miR-10b is increased in various cancers. However, the underlying mechanisms of miR-10b in the occurrence and metastasis of endometrial cancer are poorly understood. To investigate its roles and correlations with Homeobox box 3 (HOXB3) in endometrial cancer, cancer tissues and adjacent normal endometrium tissues from 20 patients with endometrial cancer were studied. miR-10b expression was significantly up-regulated (p < 0.01) in endometrial cancer tissue, whereas HOXB3 was lowly expressed. The silence of miR-10b resulted in significantly enhanced cell apoptosis, and remarkably reduced cell proliferation, migration, and invasion (p < 0.05). Moreover, the protein levels of HOXB3 were increased in KLE cells with silenced miR-10b, and dual-luciferase reporter assay suggested that miR-10b could directly target HOXB3. Furthermore, overexpression of HOXB3 promoted cell apoptosis but inhibited cell proliferation, migration, and invasion (p < 0.01). To conclude, miR-10b might control cell apoptosis, proliferation, migration, and invasion in endometrial cancer via regulation of HOXB3 expression.
微小RNA是一类与癌症的发生、发展和转移密切相关的小RNA。研究表明，miR-10b在多种癌症中表达上调。然而，miR-10b在子宫内膜癌发生和转移中的潜在机制尚不清楚。为了研究其在子宫内膜癌中的作用以及与同源盒3（HOXB3）的相关性，对20例子宫内膜癌患者的癌组织和相邻正常子宫内膜组织进行了研究。miR-10b在子宫内膜癌组织中表达显著上调（p < 0.01），而HOXB3表达较低。miR-10b沉默导致细胞凋亡显著增强，细胞增殖、迁移和侵袭明显减少（p < 0.05）。此外，miR-10b沉默的KLE细胞中HOXB3蛋白水平升高，双荧光素酶报告基因检测表明miR-10b可直接靶向HOXB3。此外，HOXB3过表达促进细胞凋亡，但抑制细胞增殖、迁移和侵袭（p < 0.01）。综上所述，miR-10b可能通过调节HOXB3表达来控制子宫内膜癌细胞的凋亡、增殖、迁移和侵袭。
