Department of Chemistry, New York University , New York, New York 10003, United States.
J Am Chem Soc. 2016 Aug 10;138(31):10047-54. doi: 10.1021/jacs.6b06508. Epub 2016 Aug 2.
Structural DNA nanotechnology combines branched DNA junctions with sticky-ended cohesion to create self-assembling macromolecular architectures. One of the key goals of structural DNA nanotechnology is to construct three-dimensional (3D) crystalline lattices. Here we present a new DNA motif and a strategy that has led to the assembly of a 3D lattice. We have determined the X-ray crystal structures of two related constructs to 3.1 Å resolution using bromine-derivatized crystals. The motif we used employs a five-nucleotide repeating sequence that weaves through a series of two-turn DNA duplexes. The duplexes are tied into a layered structure that is organized and dictated by a concert of four-arm junctions; these in turn assemble into continuous arrays facilitated by sequence-specific sticky-ended cohesion. The 3D X-ray structure of these DNA crystals holds promise for the design of new structural motifs to create programmable 3D DNA lattices with atomic spatial resolution. The two arrays differ by the use of four or six repeats of the five-nucleotide units in the repeating but statistically disordered central strand. In addition, we report a 2D rhombuslike array formed from similar components.
结构 DNA 纳米技术将分支 DNA 连接点与粘性末端内聚力相结合，从而创造出自组装的大分子结构。结构 DNA 纳米技术的主要目标之一是构建三维（3D）晶体晶格。在这里，我们提出了一种新的 DNA 基元和一种策略，该策略导致了 3D 晶格的组装。我们使用溴衍生晶体以 3.1 Å 的分辨率确定了两个相关结构的 X 射线晶体结构。我们使用的基元采用五核苷酸重复序列，该序列穿过一系列双螺旋 DNA 双螺旋。双螺旋被捆绑成层状结构，该结构由四个臂连接点的协调组织和决定；这些连接点反过来又通过序列特异性粘性末端内聚力组装成连续的阵列。这些 DNA 晶体的 3D X 射线结构有望设计新的结构基元，以创建具有原子空间分辨率的可编程 3D DNA 晶格。这两个阵列的区别在于重复但统计无序的中央链中五核苷酸单元的重复使用了四个或六个。此外，我们还报告了由类似成分形成的二维菱形阵列。
