Jansen Eugene, Viezeliene Dale, Beekhof Piet, Gremmer Eric, Ivanov Leonid
Centre for Health Protection, National Institute for Public Health and the Environment, PO Box 1, 3720 BA Bilthoven, The Netherlands.
Biochemistry Department, Medical Academy, Lithuanian University of Health Sciences, Eiveniu 4, LT-50009 Kaunas, Lithuania.
Int J Mol Sci. 2016 Jul 19;17(7):1166. doi: 10.3390/ijms17071166.
A multivitamin and mineral supplementation study of 6 weeks was conducted with male and female mice. The control group received a standard dose of vitamins and minerals of 1× the Recommended Daily Intake (RDI), whereas a second group received 3× RDI. A third group received a high dose of vitamin E (25× RDI), close to the upper limit of toxicity (UL), but still recommended and considered to be harmless and beneficial. The high dose of vitamin E caused a number of beneficial, but also adverse effects. Different biomarkers of tissue toxicity, oxidative stress related processes and inflammation were determined. These biomarkers did not change in plasma and erythrocytes to a large extent. In the liver of male mice, some beneficial effects were observed by a lower concentration of several biomarkers of inflammation. However, in the kidney of male mice, a number of biomarkers increased substantially with the higher dose of vitamin E, indicating tissue toxicity and an increased level of inflammation. Since this dose of vitamin E, which is lower than the UL, cause some adverse effects, even after a short exposure period, further studies are required to reconsider the UL for vitamin E.
对雄性和雌性小鼠进行了一项为期6周的多种维生素和矿物质补充研究。对照组接受1倍推荐每日摄入量（RDI）的标准剂量维生素和矿物质，而第二组接受3倍RDI。第三组接受高剂量维生素E（25倍RDI），接近毒性上限（UL），但仍属推荐剂量且被认为无害有益。高剂量维生素E产生了一些有益影响，但也有不良反应。测定了组织毒性、氧化应激相关过程和炎症的不同生物标志物。这些生物标志物在血浆和红细胞中变化不大。在雄性小鼠的肝脏中，几种炎症生物标志物浓度降低，观察到了一些有益影响。然而，在雄性小鼠的肾脏中，随着维生素E剂量增加，许多生物标志物大幅升高，表明存在组织毒性和炎症水平升高。由于该剂量的维生素E低于UL，即使在短暴露期后也会产生一些不良反应，因此需要进一步研究以重新考虑维生素E的UL。
